针灸通过调控凋亡因子半胱氨酸蛋白酶家族改善小鼠顺铂致肝损伤的作用机制  

Mechanism of acupuncture and moxibustion in ameliorating liver injury induced by DDP by regulating apoptosis factors Caspase-3,Caspase-8and Caspase-9

作　　者：庄语 路玫[1] 于冬冬[1,2] 王永欣 滕迎春[3] 晁利芹[4] 马田田 ZHUANG Yu;LU Mei;YU Dong-dong;WANG Yong-xin;TENG Ying-chun;CHAO Li-qin;MA Tian-tian(College of Acu-moxibustion and Tuina,Henan University of Chinese Medicine,Zhengzhou 450046,China;The Third Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450008;The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450001;Academy of Chinese Medical Sciences,Henan University of Chinese Medicine,Zhengzhou 450046)

机构地区：[1]河南中医药大学针灸推拿学院,郑州450046 [2]河南中医药大学第三附属医院,郑州450008 [3]河南中医药大学第一附属医院,郑州450001 [4]河南中医药大学中医药科学院,郑州450046

出　　处：《针刺研究》2022年第7期587-591,604,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.81072884、81804196);河南省高等学校青年骨干教师培养计划(No.2019GGJS108);河南省中医药科学研究专项课题(No.2022ZY2041);河南中医药大学2021年度研究生科研创新项目(No.2021KYCX019)。

摘　　要：目的:基于顺铂(DDP)致肝损伤模型小鼠,观察针灸后肝细胞凋亡相关的半胱氨酸蛋白酶(Caspase)的变化,探讨针灸改善肝损伤的作用机制。方法:KM小鼠随机分为空白组、模型组、针刺组和艾灸组,每组10只。采用腹腔注射DDP法复制肝损伤小鼠模型。针刺组和艾灸组分别给予针刺、艾灸“大椎”和双侧“肝俞”“肾俞”“足三里”治疗,每次6min,1次/d,连续5d。观察每组小鼠一般状况;生化法检测每组小鼠血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)、谷氨酸脱氢酶(GLDH)活性;ELISA法和Western blot法检测各组小鼠肝脏组织中Caspase-3、Caspase-8和Caspase-9含量和蛋白表达水平。结果:与空白组比较,模型组小鼠一般状况较差,肝脏组织中Caspase-3、Caspase-8、Caspase-9含量和蛋白表达及血清中AST、ALT、GLDH活性均升高(P<0.05);与模型组比较,针刺组、艾灸组小鼠一般状况改善,肝脏组织中Caspase-3、Caspase-8、Caspase-9含量和蛋白表达及血清中AST、ALT、GLDH活性均降低(P<0.05)。结论:针刺和艾灸可以通过调控小鼠肝脏组织中凋亡因子Caspase-3、Caspase-8、Caspase-9的表达并改善肝功能,从改善肝细胞凋亡的层面减轻因DDP化疗所致的肝损伤可能是针灸的作用机制之一。Objective To investigate the mechanism of the effect of acupuncture and moxibustion on improving liver injury by observing the changes of cysteine protease(Caspase)associated with hepatocyte apoptosis based on cisplatin(DDP)induced liver injury model mice.Methods Forty KM mice were randomly divided into control group,model group,acupuncture group and moxibustion group,with 10mice in each group.The liver injury model was replicated by intraperitoneal injection of DDP.In the acupuncture group and the moxibustion group,acupuncture and moxibustion were performed at“Dazhui”(GV14),and bilateral“Ganshu”(BL18),“Shenshu”(BL23),and“Zusanli”(ST36),respectively,once per day for 5d.General condition of mice in each group were observed;The activities of AST,ALT and GLDH in mice serum were detected by biochemical method.ELISA and Western blot assay were used to detect Caspase-3,Caspase-8and Caspase-9contents and protein expression in the liver tissues of each group of mice,respectively.Results Compared with the control group,the general condition of the mice in the model group was poorer,and the Caspase-3,Caspase-8and Caspase-9contents and protein expressions in liver tissues and the activities of AST,ALT and GLDH in serum were increased(P<0.05).Compared with the model group,the general condition of the mice in the acupuncture and moxibustion groups improved,and the Caspase-3,Caspase-8and Caspase-9contents and protein expressions in liver tissues and activities of AST,ALT and GLDH in serum were decreased(P<0.05).Conclusion Acupuncture and moxibustion can reduce liver injury due to DDP chemotherapy by modulating the expression of apoptotic factors Caspase-3,Caspase-8and Caspase-9in liver tissues of DDP model mice and improving liver function,which may be one of the mechanisms of the effect of acupuncture and moxibustion to ameliorates liver injury after DDP chemotherapy.

关 键 词：针刺 艾灸 顺铂 肝损伤 半胱氨酸蛋白酶 

分 类 号：R245.9[医药卫生—针灸推拿学]