艾灸“肝俞”“肾俞”对骨质疏松大鼠肌肉骨骼的影响  

Effect of moxibustion of“Ganshu”(BL18)and“Shenshu”(BL23)on musculoskeletal interaction in osteoporosis rats

作　　者：徐道明[1] 刘静[1] 朱璐 陈铭 刘兰英[1] 许华宁[1] 石雯[1] 曹雅娜 吴文忠[1] XU Dao-ming;LIU Jing;ZHU Lu;CHEN Ming;LIU Lan-ying;XU Hua-ning;SHI Wen;CAO Ya-na;WU Wen-zhong(Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属医院,南京210029

出　　处：《针刺研究》2022年第7期605-610,共6页Acupuncture Research

基　　金：江苏省卫生健康委员会科研项目(No.H2019093);江苏省研究生实践创新计划(No.SJCX21_0766)。

摘　　要：目的:观察艾灸“肝俞”“肾俞”对骨质疏松症(OP)大鼠骨密度(BMD)、生物力学、骨代谢和肌骨形态的影响,探讨“筋骨相互为用”理论在OP防治中的作用。方法:雌性SD大鼠随机分为假手术组、模型组、艾灸组和尼尔雌醇组,每组8只。采用双侧卵巢切除术建立OP大鼠模型。艾灸组予艾灸双侧“肝俞”“肾俞”,每穴15min,隔日1次,每周3次,连续12周;尼尔雌醇组予尼尔雌醇灌胃(1mg/kg),每周1次,连续12周。采用双能X线BMD测量仪测定大鼠右侧股骨和第4腰椎的BMD和骨矿质含量;三点弯曲试验检测右侧股骨的生物力学性能;ELISA法测定血清中Ⅰ型胶原C端肽(CTX-Ⅰ)、抗酒石酸酸性磷酸酶(TRACP)和雌二醇(E_(2))的含量;HE染色法观察左侧股骨和股四头肌的形态结构。结果:与假手术组比较,模型组右侧股骨和第4腰椎的BMD和骨矿质含量降低(P<0.01,P<0.05),右侧股骨载荷和刚度降低(P<0.05,P<0.01)、位移增大(P<0.01),血清CTX-Ⅰ、TRACP含量升高(P<0.01)、E_(2)含量降低(P<0.01),左侧股骨组织和股四头肌组织形态结构呈骨质疏松样改变。与模型组比较,艾灸组和尼尔雌醇组大鼠右侧股骨和第4腰椎的BMD和骨矿质含量增加(P<0.01,P<0.05,艾灸组第4腰椎骨矿质含量除外),右侧股骨的载荷和刚度升高(P<0.05,P<0.01)、位移减小(P<0.05,P<0.01),血清CTX-Ⅰ、TRACP含量降低(P<0.01,P<0.05)、E_(2)含量升高(P<0.01),两组左侧股骨和股四头肌组织形态结构明显改善。结论:艾灸“肝俞”“肾俞”可增加OP大鼠BMD和骨矿质含量,提高生物力学性能,调节骨代谢指标,改善骨及其附着的肌肉形态,提示“筋骨相互为用”是OP治疗的思路之一。Objective To observe the effect of moxibustion of“Ganshu”(BL18)and“Shenshu”(BL23)on bone mineral density(BMD),biomechanics,bone metabolism and musculoskeletal morphology of osteoporosis(OP)rats,so as to explore its mechanism underlying treatment of OP via bone-muscle interaction.Methods Thirty-two female SD rats were randomly divided into sham operation,OP model,moxibustion and medication(nilestriol)groups,with 8rats in each group.For rats of the sham operation group,a small amount of fat around the ovaries was removed,and those of the other 3groups received removal of bilateral ovaries for establishing OP model.Moxibustion was applied to bilateral BL18and BL23for 30min,once every other day,3 times a week for 12weeks.Rats of the medication group received gavage of nilestriol(1mg/kg)once a week for 12weeks.After the interventions,a dual-energy absorptiometry was used to determine the BMD and bone mineral content of the rats’right femur and the fourth lumbar vertebrae under anesthesia,and three-point bending test used to detect the biomechanical properties(including load,displacement,stiffness)of the right femur.The levels of serum type I collagen C-terminal peptide(CTX-I),acid-resistant tartrate phosphatase(TRACP)and estradiol(E_(2))were determined using enzyme-linked immunosorbent assay,and histopathological changes of the left femur and the quadriceps observed after hematoxylin-eosin(H.E.)staining.Results Compared with the sham operation group,the BMD and bone mineral contents of the right femur and the fourth lumbar vertebra,the load and stiffness of the right femur,and concentration of serum E_(2) were significantly decreased(P<0.01,P<0.05),and the displacement of the right femur,and concentrations of serum CTX-Ⅰand TRACP notably increased in the model group(P<0.01).After the interventions,the decreased levels of BMD and bone mineral contents,the load and stiffness and concentration of serum E_(2),and the increased levels of the displacement,and concentrations of serum CTX-Ⅰand TRACP were all rev

关 键 词：骨质疏松症 艾灸 生物力学 筋骨相互为用 

分 类 号：R245.81[医药卫生—针灸推拿学]