1例牛带绦虫感染的诊断和鉴定  

Diagnosis and identification of a case with Taenia saginata infection

作　　者：乔飞 刘鏊 余复昌 齐萌[1] QIAO Fei;LIU Ao;YU Fu-chang;QI Meng(College of Animal Science and Technology,Tarim University,Alar 843300,China)

机构地区：[1]塔里木大学动物科学与技术学院,阿拉尔843300

出　　处：《中国寄生虫学与寄生虫病杂志》2022年第3期285-287,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：新疆生产建设兵团中青年科技创新领军人才计划(2018CB034)。

摘　　要：塔里木大学校医院于2020年6月收治1例男性绦虫病患者,患者自诉排便时发现粪便中有乳白色蠕动的虫体节片,有绦虫病史。经服南瓜子-槟榔合剂后排出1条乳白色带绦虫。收集排出的绦虫虫体,采用体视显微镜进行形态学观察,结合流行病学资料和饮食习惯,初步鉴定为牛带绦虫。基于绦虫线粒体nad1和rrnS基因位点对虫体DNA的PCR扩增结果显示,分别扩增出约为529 bp和357 bp大小的片段。基于nad1基因位点,BLAST比对显示其与肯尼亚人源牛带绦虫(GenBank登录号AM503345)序列一致性为100%;基于rrnS基因位点,其与日本人源牛带绦虫(GenBank登录号AB031355)序列一致性为99.7%,鉴定为牛带绦虫。分别于3、6个月后对患者进行随访,患者身体状况良好,无绦虫节片排出。A male patient with taeniasis was admitted Tarim University hospital in June 2020.The patient complained that there were milky white peristaltic worm fragments in the feces and the patient reported a history of taeniasis.After administration of areca nuts and pumpkin seeds,one milky tapeworm was expelled.Morphological observation of the expelled worm was carried out under stereo microscope.Combined with epidemiological data and patient’s dietary habits,the sample was preliminarily identified as Taenia saginata infection.The gene amplification results based on the nad1 and rrnS loci of the tapeworm mitochondrion showed that fragments of 529 bp and 357 bp were successfully amplified by PCR.BLAST analysis showed that the gene sequences of the nad1 and rrnS loci had100% and 99.7% homology to T.saginata collected from human in Kenya(GenBank access number AM503345) and Japan(GenBank access number AB031355).Therefore this tapeworm was identified as T.saginata.The patients were followed up after 3 and 6 months,respectively.The patients were in good health and no tapeworm was expelled.

关 键 词：牛带绦虫 感染 诊断 鉴定 治疗 

分 类 号：R532.332[医药卫生—内科学]