二氢杨梅素诱导喉癌Hep-2细胞凋亡的机制研究  

Mechanism of dihydromyricetin-induced apoptosis of laryngeal carcinoma Hep-2 cells

作　　者：赵锐[1] 周米露 刘海[1] ZHAO Rui;ZHOU Mi-lu;LIU Hai(Department of Otolaryngology Head and Neck Surgery,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan Province,China;Department of Ophthalmology,Nanchong Central Hospital,Nanchong 637000,Sichuan Province,China)

机构地区：[1]川北医学院附属医院耳鼻咽喉头颈外科,四川南充637000 [2]南充市中心医院眼科,四川南充637000

出　　处：《中国临床药理学杂志》2022年第14期1588-1592,共5页The Chinese Journal of Clinical Pharmacology

基　　金：四川省医学科研课题计划基金资助项目(S19055)。

摘　　要：目的研究CXC趋化因子受体4(CXCR4)在二氢杨梅素诱导喉癌Hep-2细胞凋亡中的作用。方法喉癌Hep-2细胞分成对照组(0μg·mL^(-1)二氢杨梅素)、低剂量实验组(20μg·mL^(-1)二氢杨梅素)、中剂量实验组(40μg·mL^(-1)二氢杨梅素)、高剂量实验组(80μg·mL^(-1)二氢杨梅素)、高剂量实验+Vector组(80μg·mL^(-1)二氢杨梅素+pcDNA3.1)、高剂量实验+CXCR4组(80μg·mL^(-1)二氢杨梅素+pcDNA3.1-CXCR4)。以蛋白质印迹法检测CXCR4、剪切的胱天蛋白酶3(cleaved caspase-3)、糖调节蛋白78(GRP78)、CCAAT/增强子结合蛋白同源蛋白(CHOP)蛋白表达,以噻唑蓝(MTT)方法检测细胞存活率,以流式细胞术测定细胞凋亡。结果对照组、低剂量实验组、中剂量实验组、高剂量实验组、高剂量实验+Vector组、高剂量实验+CXCR4组喉癌细胞中CXCR4蛋白表达水平分别为0.58±0.04,0.45±0.04,0.36±0.06,0.28±0.03,0.27±0.05,0.53±0.08,细胞存活率分别为(100.00±7.46)%,(80.79±6.07)%,(67.33±5.55)%,(53.10±6.77)%,(52.68±6.37)%,(70.29±8.53)%,细胞凋亡率分别为(7.22±0.85)%,(9.72±0.62)%,(14.85±1.42)%,(20.70±2.17)%,(19.61±1.15)%,(14.77±1.64)%。以上指标,低、中、高剂量实验组与对照组相比,差异均有统计学意义(均P<0.05);中、低剂量实验组相比,高、中剂量实验组相比,差异均有统计学意义(均P<0.05);高剂量实验+CXCR4组与高剂量实验+Vector组相比,差异均有统计学意义(均P<0.05)。结果二氢杨梅素通过下调CXCR4调控内质网应激诱导喉癌Hep-2细胞凋亡。Objective To study the mechanism of dihydromyricetin-induced apoptosis in laryngeal carcinoma Hep-2 cells based on CXC chemokine receptor 4(CXCR4).Methods Laryngeal cancer Hep-2 cells were divided into control group(0μg·mL^(-1) dihydromyricetin),low dose experimental group(20μg·mL^(-1) dihydromyricetin),medium dose experimental group(40μg·mL^(-1) dihydromyricetin),high dose experimental group(80μg·mL^(-1) dihydromyricetin),high dose experimental+Vector group(80μg·mL^(-1) dihydromyricetin+pcDNA3.1),high dose experimental+CXCR4 group(80μg·mL^(-1) dihydromyricetin+pcDNA3.1-CXCR4).Western blot was used to detect CXCR4,cleaved cysteinyl aspartate specific proteinase 3(cleaved caspase-3),glucose regulated protein 78(GRP78),CCAAT/enhancer-binding protein C/EBP(CHOP)protein expression,methyl thiazolyl tetrazolium(MTT)method was used to detect cell survival rate,and flow cytometry was used to detect cell apoptosis.Results Expression of CXCR4 protein of control group,low dose experimental group,medium dose experimental group,high dose experimental group,high dose experimental+Vector group,high dose experimental+CXCR4 group in laryngeal cancer cells were 0.58±0.04,0.45±0.04,0.36±0.06,0.28±0.03,0.27±0.05,0.53±0.08,cell survival rates were(100.00±7.46)%,(80.79±6.07)%,(67.33±5.55)%,(53.10±6.77)%,(52.68±6.37)%,(70.29±8.53)%,the apoptosis rate were(7.22±0.85)%,(9.72±0.62)%,(14.85±1.42)%,(20.70±2.17)%,(19.61±1.15)%,(14.77±1.64)%.Above indicators,low dose experimental group,medium dose experimental group,high dose experimental group compared with control group,the difference were statistically significant(all P<0.05);compared between medium dose experimental group and low dose experimental group,compared between high dose experimental group and medium dose experimental group,the difference were all statistically significant(all P<0.05);compared between high dose experimental+CXCR4 group and high dose experimental+Vector group,the differences were all statistically significant(all P<0.05).Conclusion Di

关 键 词：二氢杨梅素 喉癌 CXC趋化因子受体4(CXCR4) 凋亡 内质网应激 

分 类 号：R28[医药卫生—中药学]