扁蒴藤素对肝细胞癌细胞Hep3B增殖和凋亡的影响  

Effect of pristimerin on proliferation and apoptosis of hepatocellular carcinoma cells Hep3B

作　　者：徐婷 程名[2] 王叙德 XU Ting;CHENG Ming;WANG Xu-de(Department of Gynecology and Surgery,Anhui Health Vocational College,Chizhou 247099,Anhui Province,China;Department of Otolaryngology,Chizhou Second People’s Hospital,Chizhou 247099,Anhui Province,China)

机构地区：[1]安徽卫生健康职业学院妇外教研室,安徽池州247099 [2]池州市第二人民医院耳鼻咽喉科,安徽池州247099

出　　处：《中国临床药理学杂志》2022年第14期1607-1611,共5页The Chinese Journal of Clinical Pharmacology

基　　金：安徽省教育厅2020年省级示范基层教学组织(教研室)基金资助项目(2020SJSFJXZZ196)。

摘　　要：目的探究扁蒴藤素对肝细胞癌细胞Hep3B增殖和凋亡的影响及机制。方法选取Hep3B细胞随机分为对照组、低浓度组、中浓度组、高浓度组、高浓度+anti-miR-NC组和高浓度+anti-miR^(-1)05-5p组。低浓度组、中浓度组和高浓度组分别用0.5,1和2μmol·L^(-1)的扁蒴藤素处理,高浓度+anti-miR-NC组和高浓度+anti-miR^(-1)05-5p组先分别转染anti-miR-NC和anti-miR^(-1)05-5p再用2μmol·L^(-1)的扁蒴藤素处理,对照组不转染也不用扁蒴藤素处理。用细胞计数法-8(CCK-8)实验检测细胞增殖,用流式细胞术检测细胞凋亡率,用实时定量逆转录聚合酶链反应(qRT-PCR)检测miR^(-1)05-5p的表达水平,用蛋白质印迹(Western blot)法检测小鼠双微体基因(MDM2)蛋白表达水平。结果对照组、高浓度组、高浓度+anti-miR-NC组和高浓度+anti-miR^(-1)05-5p组的细胞存活率分别为(100.00±0.00)%,(46.44±7.23)%,(56.23±6.64)%,(85.82±7.55)%;凋亡率分别为(3.40±0.74)%,(35.94±3.39)%,(36.29±1.58)%,(8.55±1.37)%;miR^(-1)05-5p表达水平分别为1.00±0.00,3.36±0.30,3.18±0.19,1.57±0.12;MDM2蛋白表达水平分别为0.90±0.08,0.16±0.03,0.19±0.03,0.69±0.08。上述指标,高浓度组与对照组比较,差异均有统计学意义(均P<0.05);高浓度+anti-miR^(-1)05-5p组与高浓度+anti-miR-NC组比较,差异均有统计学意义(均P<0.05)。结论扁蒴藤素可能通过miR^(-1)05-5p靶向MDM2抑制肝细胞癌细胞Hep3B增殖并诱导细胞凋亡。Objective To investigate the effect and mechanism of pristimerin on proliferation and apoptosis of hepatocellular carcinoma cells Hep3B.Methods Hep3B cells were randomly divided into control group,low concentration group,medium concentration group,high concentration group,high concentration+anti-miR-NC group and high concentration+anti-miR^(-1)05-5p group.Low concentration group,medium concentration group and high concentration group were treated with 0.5,1 and 2μmol·L^(-1) pristimerin,respectively.High concentration+anti-miR-NC group and high concentration+anti-miR^(-1)05-5p group were transfected with anti-miR-NC and anti-miR^(-1)05-5p,respectively,and then treated with 2μmol·L^(-1)pristimerin.Control group was neither transfected nor treated with pristimerin.Cell counting kit-8(CCK-8)assay was used to detect cell proliferation;flow cytometry was used to detect cell apoptosis rate;real-time quantitative polymerase chain reaction(qRT-PCR)was used to detect miR^(-1)05-5p expression level;Western blot was used to detect murine double minute 2(MDM2)protein expression level.Results The cell survival rate of control group,high concentration group,high concentration+anti-miR-NC group and high concentration+anti-miR^(-1)05-5p group were(100.00±0.00)%,(46.44±7.23)%,(56.23±6.64)%,(85.82±7.55)%,respectively;the apoptosis rate were(3.04±0.74)%,(35.94±3.39)%,(36.29±1.58)%,(8.55±1.37)%,respectively;miR^(-1)05-5p expression levels were 1.00±0.00,3.36±0.30,3.18±0.19,1.57±0.12,respectively;MDM2 protein expression levels were 0.90±0.08,0.16±0.03,0.19±0.03,0.69±0.08,respectively.There were statistically significant differences in the above indexes in high concentration group compared with control group(all P<0.05),and there were statistically significant differences in the above indexes between high concentration+anti-miR^(-1)05-5p group and high concentration+anti-miR-NC group(all P<0.05).Conclusion Pristimerin may inhibit the proliferation and induce apoptosis of hepatocellular carcinoma cells Hep3B by regul

关 键 词：扁蒴藤素 微小RNA-105-5p(miR-105-5p) 小鼠双微体基因(MDM2) 肝癌细胞 增殖 凋亡 

分 类 号：R28[医药卫生—中药学]