二氢石蒜碱减轻柯萨奇病毒B3诱导的病毒性心肌炎大鼠心肌损伤  

Dihydrolycorine reduces myocardial injury in rats with viral myocarditis induced by Coxsackieviru B3

作　　者：杨捍卫 郝谦 伍琼 YANG Han-wei;HAO Qian;WU Qiong(Department of Emergency Medicine,Taikang Tongji Wuhan Hospital,Wuhan 430000,Hubei Province,China)

机构地区：[1]泰康同济(武汉)医院急诊内科,湖北武汉430000

出　　处：《中国临床药理学杂志》2022年第14期1653-1658,共6页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究二氢石蒜碱调控内质网应激对柯萨奇病毒B3(Coxsackieviru B3,CVB3)诱导的病毒性心肌炎大鼠心肌损伤的影响。方法大鼠分成对照组、模型组(CVB3诱导病毒性心肌炎)、实验低剂量组(CVB3诱导病毒性心肌炎,10 mg·kg^(-1)二氢石蒜碱处理)、实验中剂量组(CVB3诱导病毒性心肌炎,20 mg·kg^(-1)二氢石蒜碱处理)、实验高剂量组(CVB3诱导病毒性心肌炎,40 mg·kg^(-1)二氢石蒜碱处理)、实验高剂量+衣霉素组(CVB3诱导病毒性心肌炎,40 mg·kg^(-1)二氢石蒜碱、内质网应激诱导药衣霉素处理)。以蛋白质印迹(Western blot)法检测糖调节蛋白78(GRP78)、CCAAT/增强子结合蛋白同源蛋白(CHOP)蛋白表达,检测心功能指标左心室压力最大上升速率(dp/dt_(max))、左心室射血分数(LVEF),以酶联免疫吸附(ELISA)法检测肌酸激酶同工酶(CK-MB)水平,以聚合酶链反应(PCR)法检测白细胞介素-1β(IL-1β)mRNA表达,TUNEL法检测细胞凋亡。结果对照组、模型组、实验低剂量组、实验中剂量组、实验高剂量组、实验高剂量+衣霉素组大鼠心肌组织中GRP78蛋白表达水平分别为0.23±0.05,0.48±0.04,0.40±0.03,0.33±0.02,0.25±0.02,0.39±0.03,CHOP蛋白表达水平分别为0.29±0.03,0.57±0.06,0.47±0.04,0.34±0.03,0.27±0.03,0.45±0.03,dp/dt_(max)分别为(5796.04±247.90),(3331.46±245.45),(4131.89±291.21),(4932.43±371.87),(5613.98±416.09),(4587.28±306.15)mmHg·s^(-1),LVEF分别为(72.65±7.73)%,(27.10±2.16)%,(35.13±2.12)%,(47.74±4.68)%,(60.48±5.17)%,(41.06±2.89)%,CK-MB分别为(36.21±3.32),(111.54±8.93),(83.34±8.92),(63.15±5.54),(44.88±4.17),(70.91±5.02)U·L^(-1),IL-1βmRNA表达水平分别为1.00±0.10,3.04±0.13,2.20±0.10,1.69±0.16,1.12±0.12,1.85±0.14,细胞凋亡指数分别为(1.02±0.09)%,(12.18±0.96)%,(8.59±0.63)%,(6.28±0.36)%,(4.07±0.42)%,(7.30±0.42)%,上述指标,模型组与对照组比较,差异均有统计学意义(均P<0.05);实验低剂量组、实验中剂量组、实验高剂量�Objective To study the effect of dihydrolycorine on the myocardial injury in rats with viral myocarditis induced by Coxsackieviru B3(CVB3)through inhibiting endoplasmic reticulum stress.Methods Rats were divided into control group,model group(CVB3 induced viral myocarditis),experimental low-dose group(CVB3 induced viral myocarditis,10 mg·kg^(-1) dihydrolycorine treatment),experimental medium-dose group(CVB3 induced viral myocarditis,20 mg·kg^(-1) dihydrolycorine treatment),experimental high-dose group(CVB3-induced viral myocarditis,40 mg·kg^(-1) dihydrolycorine treatment),experimental high-dose+tunicamycin group(CVB3-induced viral myocarditis,40 mg·kg^(-1) dihydrolycorine and tunicamycin treatment).Western blot method was used to detect the protein expression of glucose regulated protein 78(GRP78)and CCAAT/enhancer-binding protein C/EBP(CHOP),and the maximum left ventricular pressure(dp/dt max),left ventricular ejection fractions(LVEF)were detected as an indicator of cardiac function;enzyme linked immunosorbent assay(ELISA)method was used to detect creatine kinase MB(CK-MB);real-time polymerase chain reaction(PCR)method was used to detect interleukin-1β(IL-1β)mRNA expression;TUNEL method was used to detect cell apoptosis.Results The expression of GRP78 protein in myocardial tissue of rats in control group,model group,experimental low-dose group,experimental medium-dose group,experimental high-dose group,experimental high-dose+tunicamycin group were 0.23±0.05,0.48±0.04,0.40±0.03,0.33±0.02,0.25±0.02,0.39±0.03;CHOP protein expression were 0.29±0.03,0.57±0.06,0.47±0.04,0.34±0.03,0.27±0.03,0.45±0.03;dp/dtmax were(5796.04±247.90),(3331.46±245.45),(4131.89±291.21),(4932.43±371.87),(5613.98±416.09),(4587.28±306.15)mmHg·s^(-1);LVEF were(72.65±7.73)%,(27.10±2.16)%,(35.13±2.12)%,(47.74±4.68)%,(60.48±5.17)%,(41.06±2.89)%;CK-MB were(36.21±3.32),(111.54±8.93),(83.34±8.92),(63.15±5.54),(44.88±4.17),(70.91±5.02)U·L-1;IL-1βmRNA expression level were 1.00±0.10,3.04±0.13,2.20±0.10,1.69±0.1

关 键 词：二氢石蒜碱 病毒性心肌炎 内质网应激 心肌损伤 

分 类 号：R28[医药卫生—中药学]