皮肤致敏对TNBS诱导小鼠急性实验性结肠炎的影响  

Effect of Skin Presensitization on TNBS-induced Acute Experimental Colitis in Mice

作　　者：刘可心 王卯[1] 吴星星 吴琪 段正兰 王耀辉[1] 王琼[1] 陈玉根[1] 吴静[1,2] LIU Kexin;WANG Mao;WU Xingxing;WU Qi;DUAN Zhenglan;WANG Yaohui;WANG Qiong;CHEN Yugen;WU Jing(Affiliated Hospital of Nanjing University of Chinese medicine,Nanjing 210029,China;Chinese Medicine Institute of Nanjing University,Nanjing Drum Tower Hospital,Drum Tower Clinical Medicine College of Nanjing University of Chinese Medicine,Nanjing 210008,China)

机构地区：[1]南京中医药大学附属医院,南京210029 [2]南京大学中医研究院,南京鼓楼医院,南京中医药大学鼓楼临床医学院,南京210008

出　　处：《实验动物科学》2022年第2期23-30,共8页Laboratory Animal Science

基　　金：国家自然科学基金(81873160);2018年度院级创新发展基金专项课题(Y2018CX67);2021年江苏省研究生科研创新课题(KYCX21-1661)。

摘　　要：目的 三硝基苯磺酸(trinitrobenzenesulfonic acid, TNBS)诱导的实验性结肠炎模型中皮肤致敏是否必要尚无定论,拟探究皮肤致敏对其影响。方法 BALB/c小鼠随机分为空白(normol control, NC)组、皮肤致敏(skin presensitization, SP)组、无皮肤致敏(non-skin presensitization, NP)组,每组5只。比较两组模型及采用Luminex多功能液相芯片评估BAFF/BLys/TNFSF13B、TNF-α、ICAM-1/CD54和TIMP-1的差异。结果 与NC组比较,SP组及NP组小鼠,皮肤致敏后至灌肠前体质量均无显著差异;灌肠后SP组较NP组体质量降低(P<0.05)。NP组脾脏质量(P<0.01)及脾脏指数(P<0.05)较NC组显著降低;SP组及NP组的疾病活动指数(disease activity index, DAI)评分较NC组均显著增加(P<0.05);SP组大体结肠形态损伤指数(colon macroscopic damage index, CMDI)评分及组织病理评分较NC组均显著升高(P<0.05);BAFF/BLys/TNFSF13B显示,SP组及NP组较NC组均显著增加(P<0.01),SP组与NP组比较,差异无显著性。结论 SP组及NP组均可建立小鼠急性实验性结肠炎的模型,但使用皮肤致敏建立的急性实验性结肠炎模型成模效果更佳。Objective Whether skin presensitization is necessary in experimental colitis model induced by trinitrobenzenesulfonic acid(TNBS) has not been determined, and the effect of skin sensitization on it is investigated.Method BALB/c mice were randomly divided into normal control group(NC group), skin presensitization group(SP group), and non-skin presensitization group(NP group), 5 per group. The success of the two groups of models and the Luminex multifunctional liquid chip analysis system was used to evaluate the differential expression levels of BAFF/BLys/TNFSF13 B、TNF-α、ICAM-1/CD54 and TIMP-1 inflammatory cytokines in serum.Result Compared with the NC group, there was no significant difference in body weight between SP group and NP group from skin presensitization to before enema;After enema, the body weight of SP group was significantly lower than NP group(P<0.05). Compared with the NC group, the spleen weight(P<0.01) and spleen index(P<0.05) of the NP group were significantly reduced;the scores of disease activity index in SP group and NP group were significantly higher than the NC group(P<0.05);the colon macroscopic damage index(CMDI) in the SP group increased significantly(P<0.05);the SP group had a significant increase in histopathological score(P<0.05). Serological detection of inflammatory cytokines, the result of BAFF/BLys/TNFSF13 B showed that compared with the NC group, the SP group was increased significantly(P<0.01), but there was no significant difference between the SP group and the NP group.Conclusion Both the SP group and the NP group can successfully establish model of acute experimental colitis, but the benefit of acute experimental colitis established by skin presensitization has a better modeling effect.

关 键 词：皮肤致敏 三硝基苯磺酸 实验性结肠炎 多功能液相芯片 小鼠 

分 类 号：Q95-3[生物学—动物学]