模拟高原环境对小鼠肠道微生物菌群的影响  

Effect of Hypobaric Hypoxic Exposure on Intestinal Microbiome Composition and Community Structure in Mice

作　　者：仝永娟 束华 马刚 杨斌 刘鹏飞 李莎 丁国羽 TONG Yongjuan;SHU Hua;MA Gang;YANG Bin;LIU Pengfei;LI Sha;DING Guoyu(Department of Laboratory Animal Center,Beijing Shijitan Hospital,Beijing 100038,China)

机构地区：[1]首都医科大学附属北京世纪坛医院实验动物中心,北京100038

出　　处：《实验动物科学》2022年第2期31-37,共7页Laboratory Animal Science

基　　金：首都医科大学附属北京世纪坛医院实验室院内开放课题(2019-KF25);首都医科大学附属北京世纪坛医院院青年基金(2015-q15)。

摘　　要：目的 探索模拟高原环境低压低氧条件对小鼠肠道微生物菌群的影响。方法 将体质量均匀的6周龄SPF级雄性成年C57BL/6 N小鼠随机分为两组:常压常氧组和低压低氧组,每组6只,分别饲养于常压常氧(海拔20~60 m、氧浓度约21%)、低压低氧(模拟海拔5 000 m、氧浓度约10%)的环境中,实验周期为4周。实验结束后,采集小鼠肠道粪便样品,提取DNA,进行16 s rDNA高通量测序。结果 在门级水平上,与常压常氧组比较,低压低氧组相对丰度较高的为拟杆菌门(Bacteroidetes)和疣微菌门(Verrucomicrobia),常压常氧组相对丰度较高的为厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)等;在属级水平上,低压低氧组的瘤胃球菌属(Ruminococcus)、肠球菌属(Enterococcus)、阿克曼菌属(Akkermansia)、普雷沃氏菌UCG-001属(Prevotellaceae UCG-001)、拟杆菌属(Bacteroides)、另枝菌属(Alistipes)、狄氏副拟杆菌属(Parabacteroides)等含量增加;乳杆菌属(Lactobacillus)、别样棒菌属(Allobaculum)、瘤胃梭菌属(Ruminiclostridium)、罗斯氏菌属(Roseburia)等含量降低。其中,低压低氧组中隶属于Verrucomicrobia的Akkermansia升高的最为明显,占总含量的20.60%。结论 模拟高原环境下,小鼠肠道微生物菌群发生变化,可能与模拟高原环境中氧气含量较低且大气压较低有关。Objective To analyze the intestinal microbiome composition and community structure of normal and hypobaric hypoxic environment in mice. Method The 6-week old SPF C57 BL/6 N male mice were randomly divided into 2 groups with 6 mice in each group: the normal atmospheric pressure group(alt. 20 m to 60 m, oxygen concentration 21%) as the control test group(the CT Group), the hypobaric hypoxic group(alt. 5 000 m, oxygen concentration 10%, the HYP group). After 4 weeks, the fresh feces of the mice were collected to investigated by 16 s sequencing analysis.Result The relative abundance of Bacteroidetes and Verrucomicrobia in the feces of mice in the HYP group was higher than that in the CT group, the relative abundance of Firmicutes and Proteobacteria was lower than the CT group. The heat map result show an increase in the OTUs of the Ruminococcus, Enterococcus, Akkermansia, Prevotellaceae UCG-001, Bacteroides, Alistipes, Parabacteroides and a decrease in Lactobacillus, Allobaculum, Ruminiclostridium and Roseburia in the HYP group. Compared with the CT group, the dominant flora is Akkermansia(20.60%) of Verrucomicrobia. Conclusion The composition and community structure of intestinal microbiome is affected by the simulated conditions of altitude environment which may be related to the hypobaric hypoxic.

关 键 词：低压低氧条件 肠道微生物菌群 菌群结构 16 s rDNA测序 

分 类 号：Q95-3[生物学—动物学]