大鼠全眼球石蜡组织切片制作方法的改进  

Improved Method for Making Paraffin Slice of the Whole Rat Eyeball Tissues

作　　者：严伟明 叶倩 龙盘 陈小红 陈艳津 王雨彤 危冬昱 张作明[2] 陈梅珠 陈涛[2] YAN Weiming;YE Qian;PAN Long;CHEN Xiaohong;CHEN Yanjin;WANG Yutong;WEI Dongyu;ZHANG Zuoming;CHEN Meizhu;CHEN Tao(Department of Ophthalmology,The 900^(th)Hospital of Joint Logistic Support Force,PLA Clinical Medical College of Fujian Medical University,Dongfang Hopsital Affiliated to Xiamen University,Fuzhou 350025,China;Center of Clinical Aerospace Medicine,Air Force Military Medical University,Xi'an 710032,China;Department of Ophthalmology,The General Hospital of Western Theatre Command,PLA,Chendu 610083,China)

机构地区：[1]中国人民解放军联勤保障部队第九○○医院,福建医科大学福总临床医学院厦门大学附属东方医院,福州350025 [2]中国人民解放军空军军医大学航空航天临床医学中心,西安710032 [3]中国人民解放军西部战区总医院,成都610083

出　　处：《实验动物科学》2022年第2期87-90,共4页Laboratory Animal Science

基　　金：福建省科技基金项目(No.2020Y0076,2020J05282,2016Y0067);中国人民解放军南京军医福州总医院博士后课题(No.48678)。

摘　　要：目的探索一种制作大鼠全眼球石蜡组织切片的改进方法。方法18只SD大鼠,随机分为3组,每组6只;取双眼眼球,分别固定于4%多聚甲醛、10%中性福尔马林和混合固定液中;2 h后左眼眼球于角膜中央用注射器扎一小孔,右眼眼球使用刀片在巩膜处剖一长约0.5 cm小开口;继续固定24 h;制作石蜡组织切片,并行苏木精-伊红染色,应用光学显微镜观察全眼球石蜡组织切片制作效果。结果采用4%多聚甲醛和10%中性福尔马林组眼球皱缩变形,晶状体结构存留少,存在视网膜脱离,细胞层细胞核连续性差。混合固定液组眼球结构较完整,未见明显视网膜脱离,细胞层细胞核连续性较好;相对于左眼,右眼晶状体结构较完整。结论采用混合固定液结合眼球侧壁纵剖制作大鼠全眼球石蜡组织切片,结构较完整,方法简便,可在一定范围推广适用。Objective To explore an optimized method for making paraffin sections of whole eyeballs in rats.Method 18 SD rats were divided into three groups, with 6 in each group. Eyeballs from all three groups were enucleated and fixed in 4% paraformaldehyde, 10% neutral formalin, and a mixed fixative solution, respectively. 2 hours later, a small hole was punctured with a syringe at the cornea of the left rat eye of all groups. Meanwhile a slice of about 0.5 cm in length was cut longitudinally at the sclera of the right eye with a sharp blade. The rat eyes were then put back into the corresponding fixatives for 24 hours. The eyeballs were subjected for the whole eyeball slicing and the hematoxylin-eosin staining. Observation of the effect of eyeball tissue section was taken under microscope.Result The eyeballs fixed with 4% paraformaldehyde and 10% neutral formalin were shrank and deformed, with decreased structure of lens. The sign of the retinal detachment was found and the continuity of each nuclear layer in the retina was poor. The overall structure of the eyeball fixed in the mixed solution was relatively complete, with no shrinkage. No obvious retinal detachment occurred, and the continuity of each nuclear layer was better. Compared with the left eye, the lens structure of the right eye was more complete.Conclusion The paraffin slice of the whole rat eyeball with a longitudinal scleral section fixed in the mixed solution was relatively complete in structure. The method was simple, and could be applied in a certain range.

关 键 词：大鼠眼球 石蜡切片 固定液 解剖 

分 类 号：Q95-3[生物学—动物学]