东南景天提取物减缓人小肠上皮细胞HIEC-6辐射损伤机制的初步研究  

Mechanism of Sedum alfredii extract alleviating radiation damage in human small intestinal epithelial cells HIEC-6

作　　者：朱彤 贺俊博 武欣 王周旋 樊赛军[1] Zhu Tong;He Junbo;Wu Xin;Wang Zhouxuan;Fan Saijun(Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine,Institute of Radiation Medicine,Chinese Academy of Medical Science,Peking Union Medical College,Tianjin 300192,China)

机构地区：[1]中国医学科学院北京协和医学院放射医学研究所天津市放射医学与分子核医学重点实验室,天津300192

出　　处：《中华放射医学与防护杂志》2022年第7期493-498,共6页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金(81730086);中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-042);中央高校基本科研业务费专项资金(3332019098)。

摘　　要：目的初步确定东南景天提取物(SafE)缓解人小肠上皮细胞HIEC-6辐射损伤的机制。方法将HIEC-6细胞分为对照组(Con)、照射组(IR)、单独提取物组(SafE)、提取物照射组(SafE+IR)。SafE组使用0.02 g/ml(W/V)SafE处理24 h后,给予2、4、6 Gyγ射线照射,照后24 h检测细胞活力(CCK-8法)和细胞内活性氧(ROS)水平;4 Gy照后24 h取样进行转录组分析,检测细胞内E3泛素连接酶PRKN表达水平,使用荧光染料观察内质网,并统计内质网厚度。结果与IR组相比,SafE+IR组照射后细胞活力值显著增加(t=2.94~10.40,P<0.05);与对照组相比,SafE+IR组照射后胞内ROS水平显著降低(t=-13.29~-4.53,P<0.05)。初步筛选出SafE靶基因为PRKN;SafE可维持照射后PRKN转录水平和内质网厚度,IR组与对照组比较,差异有统计学意义(t=-5.55、3.27,P<0.05);SafE组与SafE+IR组比较,差异无统计学意义(P>0.05)。结论SafE可有效维持内质网厚度,减少细胞辐射损伤,其靶基因PRKN受电离辐射调控。Objective To confirm the mechanism of Sedum alfredii extract(SafE)alleviating radiation injury in human small intestinal epithelial cells(HIEC-6).Methods HIEC-6 cells were divided into 4 groups,including control group(Con),irradiation group(IR),SafE alone group(SafE)and SafE plus irradiation group(SafE+IR).All of the SafE groups were treated with 0.02 g/ml(W/V)SafE for 24 h.Cell viability(CCK-8 method)and intracellular ROS levels were investigated at 24 h after 2,4,and 6 Gy irradiation.Samples were taken at 24 h after 4 Gy irradiation for transcriptome analysis,and the intracellular E3 ubiquitin ligase PRKN expression level was measured.The thickness of endoplasmic reticulum was detected at 24 h after 4 Gy irradiation using fluorescent dye.Results SafE could maintain cell viability after irradiation(t=2.94-10.40,P<0.05),and significantly reduced the level of ROS in the irradiated cells(t=-13.29--4.53,P<0.05).PRKN was preliminarily verified to be the target gene of SafE that maintained PRKN transcript level and endoplasmic reticulum thickness after irradiation(IR group vs.Con group:t=-5.55,3.27,P<0.05,SafE group vs.SafE+IR group:P>0.05).Conclusion SafE is effective in maintaining ER thickness and reducing cellular radiation damage and its target gene PRKN could be regulated by ionizing radiation.

关 键 词：东南景天提取物 电离辐射损伤 PRKN 内质网 活性氧 

分 类 号：R285[医药卫生—中药学]