温度/超声双重响应型相变液滴对水凝胶结构性能的调控  

Structure and properties regulation of hydrogels by temperature/ultrasound dual-responsive phase-change droplets

作　　者：赵婵 刘昊[1] 陈洁莹 徐雪琴 胡克 段磊[1] ZHAO Chan;LIU Hao;CHEN Jieying;XUE Xueqin;HU Ke;DUAN Lei(Department of Biomedical Engineering,Nanjing Medical University,Nanjing 211166,China)

机构地区：[1]南京医科大学生物医学工程系,江苏南京211166

出　　处：《南京医科大学学报（自然科学版）》2022年第7期948-956,共9页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家重点研发计划(2019YFA0210104);国家自然科学基金(81701833);江苏省重点大学生创新创业训练计划项目(KY124J2019065)。

摘　　要：目的:制备温度/超声双重响应型脂质相变液滴(lipid phase-change droplet,LPCD),并将其与水凝胶进行复合,通过温度或低强度聚焦超声(low intensity focused ultrasound,LIFU)调控LPCD的内核发生液-气相转变,继而调控水凝胶的结构和力学性能。方法:首先通过薄膜水化法制备出脂质膜壳的全氟己烷(perfluorohexane,PFH)相变液滴,对其在温度和LIFU调控下的形貌变化、相转变能力进行表征,同时评估液-气相变前后LPCD的超声造影能力。在此基础上将其与水凝胶进行复合,通过各项表征评估温度/超声引起的液滴相变对水凝胶内部结构、浮力和机械性能的影响。结果:LPCD在温度或超声调控下有良好的相转变能力和超声造影能力。脂质液滴相变可使水凝胶内部生成直径在微米量级的诸多气孔,浮力得到显著提高,压缩应力和溶胀性能也有所变化。结论:脂质相变液滴-水凝胶复合材料(LPCD@Hy)是一种有潜力的智能响应型诊疗用微纳材料。Objective:Prepare temperature/ultrasound dual-responsive lipid phase-change droplet(LPCD)and combine them with hydrogels to regulate liquid-gas transitions in the core of lipid droplets by temperature or low intensity focused ultrasound(LIFU),and then regulate the structure and mechanical properties of the hydrogels.Methods:Firstly,perfluorohexane(PFH)phase-change droplets of lipid membrane shell were prepared by thin-film hydration method.The morphological changes and phase transition ability under the control of temperature and LIFU were characterized.At the same time,the contrast-enhanced ability of LPCD before and after liquid-gas phase transition was evaluated.On this basis,it was combined with hydrogels,and the effects of temperature/ultrasound induced droplet transition on the internal structure,buoyancy and mechanical properties of hydrogels were evaluated by characterization.Results:LPCD had good phase transition ability and contrast-enhanced ultrasound ability under the control of temperature or ultrasound.Lipid droplet phase transitions caused many micropores inside the hydrogel,which significantly improved buoyancy,varying compressive stress and swelling properties.Conclusion:Lipid phase-change droplets-hydrogel composite material(LPCD@Hy)is a kind of potential intelligent response micro/nano material for diagnosis and treatment.

关 键 词：脂质相变液滴 水凝胶 造影剂 超声成像 

分 类 号：R318.5[医药卫生—生物医学工程]