过表达羊草VHA基因提高大豆耐碱性研究  

Overexpression of LcVHA Gene Improve Alkali Tolerance in Transgenic Soybean

作　　者：任鹏飞 蔡勤安[2] 尚丽霞[2] 于志晶[2] 马瑞[2] RENG Pengfei;CAI Qińan;SHANG Lixia;YU Zhijing;MA Rui(College of Agronomy,Jilin Agricultural University,Changchun 130118;Agro-Biotechnology Research Institute/Jilin Provincial Key Laboratory of Agricultural Biotechnology,Jilin Academy of Agricultural Sciences,Changchun 130033,China)

机构地区：[1]吉林农业大学农学院,长春130118 [2]吉林省农业科学院生物技术研究所/吉林省农业生物技术重点实验室,长春130033

出　　处：《东北农业科学》2022年第3期79-82,98,共5页Journal of Northeast Agricultural Sciences

基　　金：转基因生物新品种培育重大专项(2016ZX08004002)。

摘　　要：土壤盐碱化是影响植物生长和分布、限制粮食产量的主要非生物胁迫因素。本研究通过农杆菌介导的大豆子叶节遗传转化技术将羊草LcVHA基因转入栽培大豆品种Bert中,以提高大豆耐碱性。共侵染外植体4000个,转化植株经分子检测获得了阳性植株108株,转化率为2.7%。转基因植株中外源基因拷贝数为1~5个,单拷贝率为21%。耐碱试验结果表明,转基因植株在碱胁迫(300 mmol/L NaHCO_(3))下生长较好,耐碱性和农艺性状显著高于非转基因对照植株。本研究共获得了23份耐碱转基因大豆,为耐碱转基因大豆育种提供了新材料。Soil salinization is the main abiotic stress factor that affects plant growth and distribution and limits grain yield.In this study,LcVHA gene obtained from Leymus chinensis was transferred into cultivar Bert through Agrobacterium-mediated soybean cotyledon node transformation system to improve the alkali tolerance of soybean.Together with PPT resistance selection,the transgenic plants were confirmed by PCR detection,southern blot,RT-PCR analysis and Quick PAT/bar strip detection.A total of 4,000 explants were infected.108 transgenic plants with LcVHA gene were obtained by molecular detection.The conversion rate was 2.7%.The copy number of foreign gene in transgenic plants varied from 1 to 5,and the single copy rate was 21%.The results for alkali tolerance test showed that the transgenic plants under alkali stress(300 mmol/L NaHCO_(3))grew better than the non-transgenic control plants,and the alkali tolerance of transgenic soybean was increased.In this study,total 23 alkali resistant transgenic lines were obtained,which provided new materials for the soda alkali resistant transgenic soybean breeding.

关 键 词：大豆 耐碱性 遗传转化 质子泵 

分 类 号：S565.1[农业科学—作物学]