g-C_(3)N_(4)对河床底泥细菌群落的影响  

Effects of g-C_(3)N_(4)on Bacterial Community in Riverbed Sediment

作　　者：唐瑶 胡雪梅 陈奕君 刘柳惠子 谌小勇[1,3] 赵运林[1,3] 吴耀辉[1,3] 刘曙光 TANG Yao;HU Xuemei;CHEN Yijun;LIU Liuhuizi;CHEN Xiaoyong;ZHAO Yunlin;WU Yaohui;LIU Shuguang(College of Life Science and Technology,Central South University of Forestry and Technology,Changsha 410004,China;National Engineering Laboratory for Applied Forest Ecological Technology in Southern China,Changsha 410004,China;Hunan Research Center of Engineering Technology for Utilization of Environmental and Resources Plant,Central South University of Forestry and Technology,Changsha 410004,China)

机构地区：[1]中南林业科技大学生命科学与技术学院,湖南长沙410004 [2]中国南方应用森林生态技术国家工程实验室,湖南长沙410004 [3]中南林业科技大学湖南环境资源植物利用工程技术研究中心,湖南长沙410004

出　　处：《环境科学与技术》2022年第4期1-7,共7页Environmental Science & Technology

基　　金：湖南省教育厅科研基金(19A509);国家自然科学基金(31971462);国家博士后科学基金(2020M683592,2020M682602);长沙市科技计划(Kq1907097)。

摘　　要：光催化剂对水体有机污染修复极具发展潜力,但对生态环境的影响亟待探究。为初步评估光催化剂对生态环境的影响,该研究以典型光催化剂类石墨相氮化碳(g-C_(3)N_(4))为代表,以不同g-C_(3)N_(4)投加量(0、25、50、75、100、125 mg/kg,分别记为CK、P25、P50、P75、P100及P125)处理河床底泥,30 d后,利用16S rRNA高通量测序,分析河床底泥细菌群落差异。结果表明,不同投加量的g-C_(3)N_(4)处理后,河床底泥细菌群落多样性排序为P75(g-C_(3)N_(4)投加量为75 mg/kg)>P100(g-C_(3)N_(4)投加量为100 mg/kg)>P125(g-C_(3)N_(4)投加量为125 mg/kg)>P50(g-C_(3)N_(4)投加量为50 mg/kg)>P25(g-C_(3)N_(4)投加量为25 mg/kg)>CK(g-C_(3)N_(4)投加量为0 mg/kg);在门和属水平分析河床底泥细菌群落结构,发现g-C_(3)N_(4)添加对河床底泥细菌群落结构无明显影响,但能增加河床底泥部分细菌的相对丰度,表明g-C_(3)N_(4)在一定程度上能促进细菌的生长。研究结果初步揭示了g-C_(3)N_(4)对河床底泥生态环境及细菌群落的影响,提供了初步的理论基础,并为今后g-C_(3)N_(4)在实际水体环境中的应用提供了参考。The potential of photocatalysts for the remediation of organic pollution in water bodies is extremely promising,but the ecological impact needs to be explored.To preliminarily assess the ecological impact of photocatalysts,this study used a typical photocatalyst-like graphite-phase carbon nitride(g-C_(3)N_(4))as a representative,and treated riverbed sediment with different concentrations of g-C_(3)N_(4)(0,25,50,75,100 and 125 mg/kg,named as CK,P25,P50,P75,P100 and P125 respectively)for 30 days,and then analysed the differences in the bacterial communities of riverbed substrates using16S rRNA high-throughput sequencing.The results showed that when treated by different doses of g-C_(3)N_(4),the analysis of bacterial community diversity showed that the diversity order of the six samples was P75(g-C_(3)N_(4)dosing at 75 mg/kg)>P100(g-C_(3)N_(4)dosing at 100 mg/kg)>P125(g-C_(3)N_(4)dosing at 125 mg/kg)>P50(g-C_(3)N_(4)dosing at 50 mg/kg)>P25(g-C_(3)N_(4)dosing at25 mg/kg)>CK(g-C_(3)N_(4)dosing at 0 mg/kg).The riverbed sediment bacterial community structure was analyzed at the phylum level and genus level,and it was found that the addition of g-C_(3)N_(4)had no significant impact on the bacterial community structure in riverbed sediment.However,g-C_(3)N_(4)addition increased the relative abundance of some bacteria,indicating that the application of g-C_(3)N_(4)was beneficial to the growth of bacteria.The achievements of this study could provide a preliminary theoretical basis for the influence of g-C_(3)N_(4)on ecology and bacterial communities of riverbed sediment and provide a reference for the future application of g-C_(3)N_(4)in the water environment in the future.

关 键 词：g-C_(3)N_(4) 细菌群落 高通量测序 河床底泥 

分 类 号：X522[环境科学与工程—环境工程] X172