覆盆子的化学成分研究  

Chemical constituents from Rubi Fructus

作　　者：王静宜 余俊东 陈悦 杜军 巫小宏 李医明[1] 王瑞[1] 陈亮 吴迎春[1] WANG Jing-yi;YU Jun-dong;CHEN Yue;DU Jun;WU Xiao-hong;LI Yi-ming;WANG Rui;CHEN Liang;WU Ying-chun(School of Pharmacy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Amway(Shanghai)Technology Development Co.,Ltd.,Shanghai 201203,China;Amway(China)Botanical R&D Center,Wuxi 214145,China)

机构地区：[1]上海中医药大学中药学院,上海201203 [2]安利(上海)科技发展有限公司,上海201203 [3]安利(中国)植物研发中心有限公司,江苏无锡214145

出　　处：《中草药》2022年第13期3897-3903,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81973458);上海市科委科技创新行动计划(18401931100)。

摘　　要：目的研究华东覆盆子Rubuschingii果实的化学成分。方法采用加热回流提取、溶剂萃取,硅胶柱色谱、ODS柱色谱、SephadexLH-20凝胶、半制备液相色谱等多种色谱方法进行分离纯化,并运用NMR、MS等现代谱学技术进行化合物结构鉴定。结果从华东覆盆子中共分离得到16个化合物,分别鉴定为2-(4-羟基-3-甲氧基苯基)-3-羟基丙酸丁酯(1)、1β,2α,19α-三羟基-3-羰基-12烯-乌苏烷-28-酸(2)、3β,19α-二羟基-1-羰基-齐墩果烷-12-烯-28-酸(3)、麦珠子酸(4)、negundonorin A(5)、山柰酚-3-O-刺槐二糖苷(6)、椴树苷(7)、槲皮素(8)、根皮苷(9)、5,6,7,4′-四羟基-黄酮醇(10)、香橙素(11)、2,2′,3,3′,4,4′-六羟基-6,6′-联苯二甲酸二乙酯(12)、没食子酸乙酯(13)、没食子酸(14)、(+)-儿茶素(15)、对羟基苯甲酸(16)。结论化合物1为新的酚类化合物,命名为覆盆子酚酸丁酯,化合物2~5、10为首次从该植物中分离得到。Objective To study the chemical constituents from Rubi Fructus.Methods The chemical constituents of fruits of R.chingii were isolated and purified by reflux-extraction,solvent extraction and column chromatography such as silica gel column chromatography,ODS column chromatography,Sephadex LH-20 column chromatography and semi-preparative RP-HPLC.The structures of all compounds were identified by modern spectrum technology(ESI-MS,1H-NMR,13C-NMR).Results Sixteen compounds were isolated from the fruits of R.chingii and identified as butyl-2-(4-hydroxy-3-methoxyphenyl)-3-hydroxypropanoate(1),1β,2α,19α-trihydroxy-3-oxo-12-ursen-28-oic acid(2),3β,19α-dihydroxy-1-oxo-olean-12-en-28-oic acid(3),alphitolic acid(4),negundonorin A(5),kaempferol-3-O-robinobioside(6),tiliroside(7),quercetin(8),phlorizin(9),5,6,7,4′-tetrahydroxy-flavonol(10),aromadendrin(11),diethyl-2,2′,3,3′,4,4′-hexahydroxybiphenyl-6,6′-dicarboxylate(12),ethyl gallate(13),gallic acid(14),(+)-catechin(15),and 4-hydroxybenzoic acid(16).Conclusion Among them,compound 1 is a new simple phenol named(±)-butylchinphenolate,compounds 2—5,10 are isolated from this plant for the first time.

关 键 词：覆盆子 华东覆盆子 外消旋体 覆盆子酚酸丁酯 1β 2α 19α-三羟基-3-羰基-12烯-乌苏烷-28-酸 麦珠子酸 5 6 7 4′-四羟基-黄酮醇 

分 类 号：R284.1[医药卫生—中药学]