基于HPLC指纹图谱的不同产地金钱草及其地方习用品与混用品的鉴定研究  

Study on identification technology of Lysimachia christinae and its local common articles and mixed articles based on HPLC fingerprint

作　　者：陈云 徐璐娜 王翰华 李兆慧 周漫佳 程汝滨[2] CHEN Yun;XU Lu-na;WANG Han-hua;LI Zhao-hui;ZHOU Man-jia;CHENG Ru-bin(Zhejiang Pharmaceutical University,Ningbo 315100,China;Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江药科职业大学,浙江宁波315100 [2]浙江中医药大学,浙江杭州310053

出　　处：《中草药》2022年第14期4504-4511,共8页Chinese Traditional and Herbal Drugs

基　　金：浙江医药高等专科学校校级科研基金项目(XY2020005,ZPCSR2020006,ZPCSR2020007)。

摘　　要：目的对不同产地的金钱草Lysimachia chriatinae及其常见的地方习用品与混用品的HPLC指纹图谱进行研究,比较分析不同产地金钱草及其习用品与混用品的差异,为其鉴定技术的开发应用奠定基础。方法利用80%甲醇制备金钱草及其习用品与混用品样品的提取溶液,采用HPLC法建立不同产地的金钱草及其习用品与混用品提取液的指纹图谱,明确金钱草及其地方习用品与混用品的特征鉴别峰,分析比较不同产地金钱草样品间指纹图谱的差异,利用SPSS软件对不同样品的指纹图谱进行聚类分析和主成分分析,明确HPLC指纹图谱在不同产地金钱草样品的质量评价和习用品与混用品的正伪鉴别中的作用。结果HPLC指纹图谱共获得了区分度较好的8个特征峰数据,可用于后续的鉴别和质量评价,其中金钱草和其地方习用品浙金钱草与几种混用品的指纹图谱存在显著差异,SPSS聚类分析结果表明HPLC指纹图谱可将金钱草及其地方习用品与混用品进行准确高效区分;不同产地金钱草的指纹图谱存在一定差异,其中四川产地和贵州、云南产地的可以进行有效区分。结论HPLC指纹图谱能有效区分不同产地金钱草及其地方习用品与混用品,鉴定结果具有快速、准确和操作简单的特点,为控制金钱草药材商品的质量提供了技术保障。Objective To research Lysimachia christinae and its local common articles and mixed articles based on HPLC fingerprint,and compare and analyze the differences of the medicinal materials and mixed articles in different areas,so as to lay a foundation for the development and application of the identification technology.Methods Using 80%methanol to preparation L.christinae and its local common articles and mixed articles extracts,HPLC method was used to establish the fingerprints of the Hance and its local common articles and mixed articles,clarify the characteristic identification peak of the Hance and its local common articles,analyze and compare the differences of the fingerprints of different the Hance samples.SPSS software was used for cluster analysis of fingerprints of different samples,to clarify the role of HPLC fingerprint in the quality evaluation of the Hance samples in different areas and the positive and false identification of common articles and mixed articles.Results The data of eight characteristic peaks were obtained by fingerprint.The composition and proportion of each characteristic peak in the fingerprint of different Hance species were significantly different,the results of SPSS cluster analysis showed that HPLC fingerprint could be used to accurately and efficiently distinguish the L.christinae and its local common articles.There were also some differences in the fingerprint patterns of the samples from different regions,among which the samples from Sichuan,Guizhou and Yunnan could be effectively distinguished.Conclusion HPLC fingerprint can effectively distinguish the sources of different L.christinae and its local common articles,and the identification results have the characteristics of rapid,accurate and simple operation,which provides a technical guarantee for the quality control of the L.christinae.

关 键 词：金钱草 混用品 指纹图谱 相似度 聚类分析 

分 类 号：R286.2[医药卫生—中药学]