基于免疫相关细胞因子研究理冲汤对子宫肌瘤细胞凋亡和增殖的影响  

Effects of Lichong Decoction on Apoptosis and Proliferation of Uterine Fibroid Based on Immune-related Cytokines

作　　者：李冬华[1] 刘宇[1] 王文娜 LI Donghua;LIU Yu;WANG Wenna(School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学中医药学院,北京100069

出　　处：《世界中医药》2022年第12期1692-1696,共5页World Chinese Medicine

基　　金：国家自然科学基金项目(81774072)——从IDO介导的免疫编辑途径研究理冲汤抑制子宫肌瘤免疫逃逸的作用及分子机制;北京市自然科学基金项目(7202015)——基于LncRNA调控信号通路研究理冲汤对子宫肌瘤血管生成/抑制因子平衡漂移的影响。

摘　　要：目的:从免疫相关细胞因子表达情况研究理冲汤对子宫肌瘤细胞凋亡和增殖的影响。方法:用异种移植法建立子宫肌瘤小鼠模型,随机分为正常组、模型组、理冲汤组和阳性药组,每组6只,连续药物干预4周;通过透射电子显微镜观察子宫平滑肌细胞的超微结构;采用免疫组织化学染色检测促进细胞凋亡因子胱天蛋白酶3(Caspase-3)、抑制细胞增殖因子Ki67的表达情况;采用MSD电化学发光技术检测白细胞介素-12p70(IL-12p70)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤生长相关基因(GRO/KC)的表达情况。结果:与模型组比较,理冲汤组子宫平滑肌细胞超微结构改善明显,细胞排列紊乱和细胞膜增厚现象均有所减轻;子宫组织中Caspase-3表达升高(P<0.01),Ki67表达降低(P<0.01);血清中IL-12p70表达升高(P<0.05),IL-1β、IL-6、GRO/KC表达降低(P<0.05,P<0.01)。结论:理冲汤具有促进子宫肌瘤细胞凋亡、抑制细胞增殖的作用,其分子机制与调控免疫相关细胞因子表达有关。Objective:To investigate the effects of Lichong Decoction on the apoptosis and proliferation of uterine fibroid based on the expression of immune-related cytokines.Methods:The uterine fibroid model was induced in mice by xenotransplantation,and the model mice were randomly divided into a model group,a Lichong Decoction group,and a positive drug group,with six mice in each group.Six normal mice were assigned to the normal group.Drug intervention was conducted for four weeks.The ultrastructure of uterine smooth muscle cells was observed by transmission electron microscopy.The expression of Caspase-3 and Ki67 was detected by immunohistochemistry.The expression of IL-12p70,IL-1β,IL-6,and growth-related oncogene(GRO/KC)was detected by Meso Scale Discovery(MSD)-electrochemiluminescence method.Results:Compared with the model group,the Lichong Decoction group showed improved ultrastructure of uterine smooth muscle cells,ameliorative cell arrangement and the thickening of the cell membrane,increased expression of Caspase-3 in uterine tissues(P<0.01),decreased expression of Ki67(P<0.01),elevated expression of IL-12p70 in the serum(P<0.05),and reduced expression of IL-1β,IL-6,and GRO/KC(P<0.05,P<0.01).Conclusion:Lichong Decoction can promote the apoptosis of uterine fibroid and inhibit its cell proliferation and its molecular mechanism may be related to the regulation of the expression of immune-related cytokines.

关 键 词：子宫肌瘤 理冲汤 细胞凋亡 细胞增殖 免疫细胞因子 半胱氨酸蛋白水解酶3 白细胞介素-12p70 

分 类 号：R289.4[医药卫生—方剂学] R711.74[医药卫生—中药学]