全缘兔耳草不同萃取部位黄酮和环烯醚萜苷含量及其抗氧化和抗AChE活性研究  

Study on flavonoid and iridoid glycoside contents of different extraction sites of Lagotis integra and its antioxidant and AChE inhibitory activities

作　　者：李楚研 王润坤 邹美佳 秦娟 刘量 LI Chuyan;WANG Runkun;ZOU Meijia;QIN Juan;LIU Liang(Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases/College of Medicine(Institute of Translational Medicine),Yangzhou University,Yangzhou 225009,China;Pharmacy Department of Xuyi People's Hospital,Xuyi 211700,China)

机构地区：[1]扬州大学医学院(转化医学研究院)/江苏省中西医结合老年病防治重点实验室,江苏扬州225009 [2]盱眙县人民医院药剂科,江苏盱眙211700

出　　处：《扬州大学学报（农业与生命科学版）》2022年第3期92-96,107,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏高校“青蓝工程”项目(20200104);扬州大学“高端人才支持计划”项目(20190223);扬州大学“青蓝工程”项目(20180210)。

摘　　要：为研究全缘兔耳草不同萃取部位黄酮和环烯醚萜苷含量及其抗氧化和抗乙酰胆碱酯酶(acetylcholinesterase,AChE)活性,采用紫外可见分光光度法测定全缘兔耳草不同萃取部位黄酮和环烯醚萜苷含量。以加兰他敏为阳性对照,采用AChE抑制试验,评价不同萃取部位AChE抑制活性。以维生素C为阳性对照,采用DPPH和ABTS法评价不同萃取部位抗氧化活性。结果表明:乙酸乙酯部位和正丁醇部位黄酮和环烯醚萜苷含量显著高于石油醚部位;乙酸乙酯部位和正丁醇部位抗氧化和抗AChE活性也显著高于石油醚部位。皮尔森相关分析表明,全缘兔耳草黄酮和环烯醚萜苷含量与抗氧化和AChE抑制活性呈显著的正相关,提示黄酮类和环烯醚萜苷类成分可能是全缘兔耳草发挥抗氧化和抑制AChE作用的活性成分。The contents of flavonoids and iridoid glycosides of different extraction sites of Lagotis integra and its antioxidant and acetylcholinesterase inhibitory activities were studied.An UV-visible spectrophotometry was used to measure the flavonoid and total iridoid glycoside contents of different extraction sites of L.integra.With galanthamine as a positive control,an acetylcholinesterase inhibition experiment was used to evaluate the acetylcholinesterase inhibitory activity of different extraction sites.With Vitamin C as a positive control,DPPH and ABTS assays were carried out to evaluate the antioxidant activities of different extraction sites.The results showed that the contents of flavonoids and iridoid glycosides of ethyl acetate and n-butanol parts were significantly higher than those of petroleum ether.In addition,the antioxidant and acetylcholinesterase inhibitory activities of ethyl acetate and n-butanol parts were also significantly stronger than those of petroleum ether part.Results of Pearson correlation analysis showed that there were significant positive correlations between the contents of flavonoids/iridoid glycosides and antioxidant/acetylcholinesterase inhibitory activities,indicating that flavonoids and iridoid glycosides may be the active constituents,which exert the antioxidant and acetylcholinesterase inhibitory activities of L.integra.

关 键 词：全缘兔耳草 黄酮 环烯醚萜苷 乙酰胆碱酯酶抑制活性 抗氧化活性 

分 类 号：R284[医药卫生—中药学]