CAZy-AA3家族酶及其在生物传感器中的应用研究进展  

Research Progress of CAZy-AA3 Family Enzymes and Their Applications in Biosensors

作　　者：张振宇 公维丽 马耀宏 朱思荣 王丙莲 韩庆晔 陈彦儒 ZHANG Zhen-Yu;GONG Wei-Li;MA Yao-Hong;ZHU Si-Rong;WANG Bing-Lian;HAN Qing-Ye;CHEN Yan-Ru(Biology Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250103,China)

机构地区：[1]齐鲁工业大学(山东省科学院)生物研究所,济南250103

出　　处：《生物化学与生物物理进展》2022年第7期1192-1207,共16页Progress In Biochemistry and Biophysics

基　　金：齐鲁工业大学生物及生物化学ESI培育学科基金(ESIBBC202010)资助项目。

摘　　要：碳水化合物活性酶数据库(CAZy)中位于“辅助活性”(auxiliary activities,AA) 3家族的酶属于葡萄糖-甲醇-胆碱氧化还原酶大家族。它们以黄素腺嘌呤二核苷酸(FAD)作为辅酶,通过反应产物(H2O2或对苯二酚)协助其他AA家族酶发挥作用,或辅助糖苷水解酶降解木质纤维素。根据结构序列相似性,AA3家族酶进一步细分为4个亚家族,包括AA3_1 (纤维二糖脱氢酶)、AA3_2 (芳醇氧化酶、葡萄糖氧化还原酶)、AA3_3 (醇氧化酶)、AA3_4 (吡喃糖氧化还原酶)。AA3家族酶因其独特的结构、广泛的用途,近几十年来受到人们的广泛关注。本文系统综述了CAZy-AA3家族酶来源、分子结构及改造,对部分AA3家族酶在生物传感器中的最新研究进展进行了重点综述,并对未来研究方向进行了展望。Enzymes in the “Auxiliary Activities”(AA) 3 family in the Carbohydrate-Active enZYmes Database(CAZy) belong to the glucose-methanol-choline family. All the members in AA3 family use flaxin-adenine dinucleotide(FAD) as a cofactor, assisting enzymes from other AA families to perform their functions via their reaction products(H2O2or hydroquinone), or facilitating glycoside hydrolase to degrade lignocellulose. According to the structure and sequence similarity, the enzymes from AA3 family were further subdivided into 4 subfamilies,mainly including cellobiose dehydrogenase, aryl-alcohol oxidase and glucose oxidoreductase, alcohol oxidase,pyranose oxidoreductase. On account of the high expression level, efficient catalysis, diversity of biotransformation, and fast electron transport kinetics, AA3 enzymes have become an interesting target for electrochemical biosensors. In order to improve the performance parameters of biosensors, a tremendous development has been obtained in the field ranging from the production of new AA3 enzymes with tailor-designed properties, such as the improved specific activity, low O2sensitivity and enhanced redox mediator interaction, to their efficient immobilization in a variety of nanomatrices. Thus in this paper, we provide an overview of the phylogenetic, molecular, and catalytic properties of CAZy-AA3 family enzymes, and the latest research progress of AA3 enzymes used in electrochemical biosensors was also summarised. In the development trend of the future study, it calls for the combination of protein engineering skills with expertise on redox mediator/polymer synthesis and electrochemistry to facilitate the application of AA3 enzymes in biosensor.

关 键 词：AA3家族酶 黄素腺嘌呤二核苷酸(FAD) 亚家族 生物传感器 

分 类 号：Q5[生物学—生物化学] Q55