Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat  

作　　者：CRISTIAN ACOSTA MABEL FOSCOLO JUAN CARLOS CAVICCHIA 

机构地区：[1]Instituto de Histología y Embriología de Mendoza(IHEM)-CONICET,Universidad Nacional de Cuyo,5500,Mendoza,Argentina

出　　处：《BIOCELL》2017年第2期63-65,共3页生物细胞（英文）

摘　　要：The testis is a double gland comprised of the sperm-producing seminiferous tubules and a complex endocrine interstice.The former structures generate and release whole cells(exocrine aspect of the gland)while the latter synthesize and release androgens and related hormones.The testis also has poorly understood paracrine connexions.These connexions play an important role during spermatogenesis.A key molecule within these structures is β-actin.Therefore,the present study aims at examining the expression pattern of β-actin during the various stages of the spermatic cycle in the rat.To achieve this goal,we used a combination of trans-illumination assisted microdissection of seminiferous tubules,confocal immunofluorescence and Western blot analysis.Our experiments revealed that the levels of β-actin fluctuate significantly during spermatogenesis,peaking immediately after spermiation.

关 键 词：Beta actin Seminiferous epithelium Trans-illumination assisted microdissection 

分 类 号：R33[医药卫生—人体生理学]