维生素D基因FokⅠ位点多态性与前列腺增生中核因子κB表达相关性及意义  

Relationship between SNP of VDR gene FokⅠ polymorphism and expression of NF-κB in BPH

作　　者：杨国尚 阮黎 华兴 YANG Guo-shang;RUAN Li;HUA Xing(Jinan University,Guangzhou 510000 China;Department of Urology,Guangzhou Red Cross Hospital,Guangzhou Red Cross Hospital Affiliated to Medical College of Jinan University,Guangzhou 510000,China;Department of Pathology,Guangzhou Red Cross Hospital,Guangzhou 510000,China)

机构地区：[1]暨南大学,广东广州510000 [2]广州市红十字会医院暨南大学医学院附属广州红十字会医院泌尿外科,广东广州510000 [3]广州市红十字会医院病理科,广东广州510000

出　　处：《吉林医学》2022年第8期2021-2024,共4页Jilin Medical Journal

基　　金：广州市医药卫生科技项目[项目编号:20161A011021];广州市卫生健康科技项目[项目编号:20181A010018]。

摘　　要：目的:探讨维生素D(VDR)基因FokⅠ位点多态性与前列腺增生(BPH)中核因子κB(NF-κB)表达相关性及意义。方法:收集511例手术治疗的BPH患者的血液及组织标本。应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)对上述血液样本进行FokⅠ多态性检测,通过免疫组化方法分析BPH组织标本中NF-κB表达情况。分析VDR不同基因型与NF-κB表达的相关性。结果:NF-κB阳性表达中三种基因型FF、Ff以及ff分布情况为82例(20.7%)、176例(44.3%)、139例(35.0%),NF-κB阴性表达中三种基因型的分布情况:38例(33.3%),49例(43.0%),27例(23.7%),两种表达情况下三种基因型的分布情况差异均无统计学意义(P>0.05),分布符合Hardy-Weinberg定律。FF、Ff以及ff三种基因型中NF-κB阳性率分别为82例(68.3%)、176例(78.2%)、139例(83.7%),阴性率分别38例(31.7%)、49例(21.8%)、27例(16.3%),差异有统计学意义(P<0.05),结论:VDR基因FokⅠ多态性与NF-κB的表达有相关性。Objective To investigate the relationship between single nucleotide polymorphism(SNP) of vitamin D receptor(VDR) gene and expression of NF-κB in BPH.Method The DNA of 511 patients with BPH were abstracted from blood respectively.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was used in determining VDR genotypes.The expression of NF-κB were detected by immunohistochemistry in BPH.The relationship was evaluated between VDR gene polymorphism of FokⅠ alleles and expression of NF-κB.Results Genotype distribution of FF,Ff,ff in group NF-κB positive was 20.7%(82/397),44.3%(176/397),35.0%(139/397)respectively and 33.3%(38/114),43.0%(49/114),23.7%(27/114)in group NF-κB negative.The genotype distribution of two group was in Hardy-Weinberg equilibrium(P>0.05).The ratio of NF-κB positive in FF,Ff and ff genotype was 68.3%(82/120),78.2%(176/225),83.7%(139/166)respectively.The ratio of NF-κB negative in FF,Ff and ff genotype was 31.7%(38/120),21.8%(49/225),16.3%(27/166)respectively.There was significant difference between genotype of VDR and expression of NF-κB(P<0.05).Conclusion VDR FokⅠpolymorphisms is correlated with expression of NF-κB in BPH.

关 键 词：前列腺增生 单核苷酸多态性 维生素D受体 NF-κB 

分 类 号：R699.8[医药卫生—泌尿科学]