梅花鹿成纤维细胞因子受体2基因多态性及其与茸重性状的关联分析  

Analysis of FGFR2 Gene Polymorphism and Their Correlation with Antler Weight Traits in Sika Deer

作　　者：周雅 张禾垟 刘琳玲[1] 李浩东 郑军军[1] 王桂武[1] ZHOU Ya;ZHANG Heyang;LIU Linling;LI Haodong;ZHENG Junjun;WANG Guiwu(Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences,Changchun 130112,China)

机构地区：[1]中国农业科学院特产研究所,长春130112

出　　处：《中国畜牧兽医》2022年第8期3006-3014,共9页China Animal Husbandry & Veterinary Medicine

基　　金：中国农业科学院科技创新工程项目(CAAX-ASTIP-2016-USAOS02)。

摘　　要：【目的】探索梅花鹿成纤维细胞因子受体2(fibroblast growth factor receptor 2,FGFR2)基因多态性及其对茸重性状的影响。【方法】应用直接测序法对梅花鹿FGFR 2基因的全部外显子进行测序分析,通过MassARRAY SNP分型技术对314头24月龄梅花鹿进行基因分型和单倍型分析,分析FGFR 2基因不同基因型和单倍型与茸重的关联性。【结果】在梅花鹿FGFR 2基因中共发现12个多态性位点,其中5个位于外显子区域,且突变均未引起氨基酸改变,属于同义突变,其余7个位点均存在于内含子区域。分型结果显示,g.80975864 T>G位点未分型成功,后续对其余11个位点进行了分析,g.80943673 T>C、g.80943683 C>A及g.80938352 C>T 3个位点属于中度多态位点(0.25<P<0.5),其余位点均属于低度多态位点(P<0.25)。χ^(2)检验结果表明,g.80998742 G>A和g.80987708 G>A 2个位点偏离Hardy-Weinberg平衡(P<0.05),其他9个位点均处于Hardy-Weinberg平衡(P>0.05)。关联分析结果表明,11个多态性位点各基因型之间的茸重差异均不显著(P>0.05)。单倍型结果显示,FGFR 2基因存在5种单倍型,不同单倍型间梅花鹿茸重差异均不显著(P>0.05)。【结论】FGFR 2基因的11个突变位点可能不是影响梅花鹿茸重性状的关键位点。【Objective】This study was performed to explore the polymorphism of fibroblast growth factor receptor 2(FGFR2)gene in sika deer and its effect on antler weight traits.【Method】All exons of FGFR2 gene of sika deer were sequenced and analyzed by direct sequencing.31424-month-old sika deer were genotyped and haplotyped by MassARRAY SNP typing technology,and the correlation between different genotypes and haplotypes of FGFR2 gene and antler weight traits was analyzed.【Result】A total of 12 SNPs were found in the FGFR2 gene in sika deer,of which 5 were located in the exon region,and none of the mutations caused amino acid changes,belonging to synonymous mutations,and the other 7 SNPs were located in the intron region.The typing results showed that g.80975864 T>G site was not successfully typing,and the remaining 11 SNPs were subjected to subsequent analysis.The 3 SNPs of g.80943673 T>C,g.80943683 C>A and g.80938352 C>T belonged to moderately polymorphic(0.25<PIC<0.5),and the rest belonged to low-polymorphism(PIC<0.25).χ^(2)fitness test results showed that the 2 SNPs of g.80998742 G>A and g.80987708 G>A deviated from Hardy-Weinberg equilibrium(P<0.05),and the others were in Hardy-Weinberg equilibrium.(P>0.05).The results of association analysis showed that there was no significant difference in antler weight among the genotypes of the 11 SNPs(P>0.05).Haplotype results showed that there were five haplotypes in FGFR2 gene,and there was no significant difference between different haplotypes and antler weights(P>0.05).【Conclusion】The 11 loci at FGFR2 were not the potential markers for antler weight.

关 键 词：梅花鹿 成纤维细胞因子受体2(FGFR2)基因 SNP 鹿茸重量 

分 类 号：S825[农业科学—畜牧学]