中药联合益生菌对大肠杆菌性小鼠腹泻的保护作用  

Protective Effect of Traditional Chinese Medicine Combined with Probiotics on E.coli Diarrheain Mice

作　　者：李春亭 王沙沙[1] 赵欣 王巍[1] 葛冰洁 张雪梅[1] LI Chunting;WANG Shasha;ZHAO Xin;WANG Wei;GE Bingjie;ZHANG Xuemei(Agriculture College of Yanbian University,Yanji 133002,China)

机构地区：[1]延边大学农学院,延吉133002

出　　处：《中国畜牧兽医》2022年第8期3226-3234,共9页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(32060817);吉林省科技发展计划项目(20210202034NC);吉林省第十七批创新创业人才资助项目(2021Y017)。

摘　　要：【目的】研究中药联合益生菌对大肠杆菌性小鼠腹泻的保护作用。【方法】通过正交试验筛选中药联合益生菌最佳因素配比。取60只小鼠随机分为空白组、模型组、中药组、益生菌组和中药联合益生菌组,每组12只。空白组小鼠腹腔注射生理盐水(0.2 mL/只),模型组、中药组、益生菌组和中药联合益生菌组均先腹腔注射分离菌悬液1×10^(7)CFU/kg,然后中药组按10 mL/kg灌胃中药液(其中蒲公英提取物浓度为0.25 g/mL,黄芪总黄酮和黄芪多糖浓度均为0.05 g/mL),益生菌组按4 mL/kg灌胃枯草杆菌悬液(菌含量5×10^(7)CFU/mL),中药联合益生菌组按上述量灌胃中药液和枯草杆菌混合液,每天定时灌胃给药1次,连续3 d。每日记录小鼠腹泻、体重、采食量等情况。末次给药12 h后分离心脏、肝脏、肾脏、脾脏、肺脏,称重并计算脏器系数;血液分析法检测血液学指标;取粪便及小肠内容物检测粪便隐血情况并进行细菌计数;ELISA法检测小鼠血清和小肠组织中髓过氧化物酶(MPO)活性。【结果】中药联合益生菌最佳因素配比为ABCD。与空白组相比,模型组小鼠腹泻率100%且无自愈现象,症状和剖检病变明显,腹泻指数极显著上升(P<0.01),体重、采食量均极显著下降(P<0.01),肝脏、脾脏器官指数均显著升高(P<0.05),淋巴细胞比率(LYM)、中间细胞比率(MID)均显著下降(P<0.05),粒细胞比率(GRAN)极显著上升(P<0.01);与模型组相比,中药组和中药联合益生菌组腹泻指数极显著下降(P<0.01),体重、采食量显著上升(P<0.05),中药组肝脏、脾脏器官指数显著下降(P<0.05),中药联合益生菌组脏器系数极显著下降(P<0.01),中药组、益生菌组及中药联合益生菌组LYM显著上升(P<0.05)、GRAN显著下降(P<0.05)。粪便隐血检测结果显示,模型组检测呈强阳性;中药组、益生菌组呈弱阳性,中药联合益生菌组呈阴性。与空白组相比,模型组小鼠肠内大肠�【Objective】The purpose of this experiment was to study the protective effect of traditional Chinese medicine(TCM)combined with probiotics on E.coli diarrhea in mice.【Method】The best factor ratio of TCM combined with probiotics was selected by orthogonal test.60 mice were randomly divided into blank group,model group,TCM group,probiotics group and TCM combined probiotics group,with 12 mice in each group.Mice in blank group were intraperitoneally injected with normal saline(0.2 mL/mouse),model group,TCM group,probiotics group and TCM combined probiotics group were intraperitoneally injected with E.coli isolate 1×10^(7)CFU/kg.The TCM group was gavaged with mixed TCM solution for 10 mL/kg(the concentration of dandelion extract was 0.25 g/mL,and the concentration of total flavonoids of Astragalus membranaceus and Astragalus polysaccharides were 0.05 g/mL),the probiotic group was gavaged with Bacillus subtilis suspension for 4 mL/kg(Bacillus subtilis contents 5×10^(7)CFU/mL),the TCM combined with probiotics group was gavaged with the mixture of TCM and Bacillus subtilis,they were given by gavage once a day for 3 days.The diarrhea,body weight and dietary quantity of the mice were recorded every day.12 hours after the last administration,the heart,liver,kidney,spleen and lung were separated,weighed and the viscera coefficient were calculated.Hematological indicators were analyzed by blood test.Fecal and small intestine contents were taken to observe fecal occult blood and count bacteria.The activity of MPO in serum and small intestine were detected by ELISA.【Result】The best factor ratio of TCM combined with probiotics was ABCD.The results showed that compared with blank group,the diarrhea rate of the model group was 100%without self-healing and there were obvious symptoms and pathological changes.The diarrhea index was extremely significantly increased(P<0.01),body weight and dietary amount were extremely significantly decreased(P<0.01),liver and spleen indexes were increased(P<0.05),the lymphocyte ratio an

关 键 词：中药联合益生菌 细菌性腹泻 小鼠 大肠杆菌 

分 类 号：S859.7[农业科学—临床兽医学]