硅对盐胁迫下黄瓜生长和多胺代谢的影响  

Effects of silicon on growth and polyamine metabolism in cucumber under salt stress

作　　者：秦曼丽 胡绪峰 刘德麒 贾建华 朱雄萌 杨静 宫海军[2] 尹军良 朱永兴 QIN Manli;HU Xufeng;LIU Deqi;JIA Jianhua;ZHU Xiongmeng;YANG Jing;GONG Haijun;YIN Junliang;ZHU Yongxing(College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei 434025,China;College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China;Vocational Institute of Engineering,Chongqing 402260,China)

机构地区：[1]长江大学园艺园林学院,湖北荆州434025 [2]西北农林科技大学园艺学院,陕西杨凌712100 [3]重庆工程职业技术学院,重庆402260

出　　处：《植物生理学报》2022年第6期1077-1091,共15页Plant Physiology Journal

基　　金：湖北省教育厅科研计划项目(D20201301)。

摘　　要：硅可缓解植物的盐胁迫损伤,但不同植物中硅提高植物抗盐性的机理不同。以黄瓜为试材,采用营养液水培方式,研究了外源加硅对盐胁迫下黄瓜幼苗生长、氧化损伤和多胺代谢的影响。结果表明施硅可改善盐胁迫下黄瓜幼苗的生长,提高叶绿素含量和PSII最大光化学量子产量(Fv/Fm)。加硅可降低盐胁迫下黄瓜中游离腐胺(Put)的含量,增加亚精胺(Spd)和精胺(Spm)的含量,同时降低二胺氧化酶(DAO)和多胺氧化酶(PAO)等多胺降解酶的活性。此外,外源施加硅、Spd和Spm可降低超氧化物歧化酶(SOD)、过氧化物酶(POD)的活性,提高过氧化氢酶(CAT)的活性,降低叶片电解质渗透率、丙二醛(MDA)以及过氧化氢(H_(2)O_(2))的含量,减轻盐胁迫造成的氧化损伤。探讨硅提高黄瓜抗盐性的机制,可为硅肥的应用提供理论依据。Silicon can alleviate the salt stress damage of plants,but its mechanism of improving salt resistance of plants is variant in different plants.Using cucumber as experimental material,the effects of exogenous silicon on the growth,oxidative damage and polyamine metabolism of cucumber seedlings under salt stress were studied.The results showed that the application of silicon could improve the growth of cucumber seedlings under salt stress,increase the content of chlorophyll and the maximum photochemical quantum yield of PSII(F_(v)/F_(m)).Silicon could decrease the content of free putrescine(Put),increase the content of spermidine(Spd)and spermine(Spm),and decrease the activities of polyamine degrading enzymes such as diamine oxidase(DAO)and polyamine oxidase(PAO)in cucumber under salt stress.In addition,exogenous application of silicon,Spd and Spm decreased the activities of superoxide dismutase(SOD)and peroxidase(POD),increased the activity of catalase(CAT),decreased the rate of electrolyte leakage,the content of malondialdehyde(MDA)and hydrogen peroxide(H_(2)O_(2))in leaves,and alleviated the oxidative damage caused by salt stress.Discussion on the mechanism of silicon improving salt resistance of cucumber can provide theoretical basis for the application of silicon fertilizer.

关 键 词：黄瓜 硅 多胺代谢 盐胁迫 

分 类 号：S642.2[农业科学—蔬菜学]