八仙花一步成苗离体培养方法  

In vitro regeneration of one-step seedling formation for Hydrangea macrophylla

作　　者：闫学彤 郑珂媛 王可佳 周慧晶 陈海霞 朱木兰[1,2,4] YAN Xuetong;ZHENG Keyuan;WANG Kejia;ZHOU Huijing;CHEN Haixia;ZHU Mulan(Shanghai Key Laboratory of Plant Functional Genomics and Resources,Shanghai Chenshan Botanical Garden,Shanghai 201602,China;Shanghai Key Laboratory of Plant Molecular Sciences,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;Hunan Mid-subtropical Quality Plant Breeding and Utilization Engineering Technology Research Center,College of Horticulture,Hunan Agricultural University,Changsha 410128,China;CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China)

机构地区：[1]上海辰山植物园,上海市资源植物功能基因组学重点实验室,上海201602 [2]上海师范大学生命科学学院,上海市植物分子科学重点实验室,上海200234 [3]湖南农业大学园艺学院,湖南省中亚热带优质花木繁育与利用工程技术研究中心,长沙410128 [4]中国科学院分子植物科学卓越创新中心,上海200032

出　　处：《植物生理学报》2022年第6期1153-1160,共8页Plant Physiology Journal

基　　金：上海市绿化和市容管理局科技攻关项目(G212403)。

摘　　要：八仙花(Hydrangea macrophylla)花序硕大,花色多样,变种多,观赏价值高,国内外广泛栽培。本研究开展了八仙花的一步成苗离体培养及组织器官激素富集研究。结果表明:MS+1.0 mg·L^(-1)6-BA+0.2mg·L^(-1)NAA为八仙花茎段一步成苗诱导最佳配方,首次建立了八仙花一步成苗离体再生技术体系。八仙花受试组织器官6-BA富集水平依次为叶片>茎段>叶柄>茎尖,NAA富集水平依次为叶片>叶柄>茎尖>茎段。本实验在同一培养基条件下,受试材料活力高,无需更换培养基,由外植体一次性发育为完整植株,操作简单,成本低廉,适宜规模化生产。Hydrangea macrophylla with huge inflorescences and various colors has high ornamental value and is widely cultivated all over the world.This study carried out the one-step seedling culture of hydrangea in vitro and the characteristics of hormone enrichment in different tissue organs.The results showed that MS+1.0 mg·L^(-1) 6-BA+0.2 mg·L^(-1) NAA was an excellent one-step seedling formation induction formula,with stems of hydrangea as explants.This research established a technical system for one-step regeneration of hydrangea seedlings in vitro for the first time.The concentration of 6-BA in the tested tissues and organs of hydrangea was leaf>stem>petiole>stem tip,and the NAA enrichment level was leaf>petiole>stem tip>stem.Under the same culture medium condition,the test material had high vitality,no need to change the culture medium,and the explant grew into a complete plant at one step.The operation is convenient and it is suitable for large-scale production.

关 键 词：八仙花 离体再生 一步成苗 激素富集 

分 类 号：S685.99[农业科学—观赏园艺]