噪声性耳聋大鼠下丘和腹外侧眶皮层fMRI局部一致性与焦虑抑郁样行为的关系  

The relationship between anxiety-and depression-like behavior and regional homogeneity of fMRI in inferior colliculus and ventrolateral orbital cortex in rats with noise-induced hearing loss

作　　者：徐晓敏[1] 陈宇辰[1] 胡京华 徐进敬[2] 高隽 殷信道[1] Xu Xiaomin;Chen Yuchen;Hu Jinghua;Xu Jinjing;Gao Jun;Yin Xindao(Department of Radiology,Nanjing First Hospital,Nanjing Medical University,Nanjing 210006,China;Department of Otolaryngology,Nanjing First Hospital,Nanjing Medical University,Nanjing 210006,China;Department of Neurobiology,Key Laboratory of Human Functional Genomics of Jiangsu,Nanjing Medical University,Nanjing 211166,China)

机构地区：[1]南京医科大学附属南京医院(南京市第一医院)医学影像科,南京210006 [2]南京医科大学附属南京医院(南京市第一医院)耳鼻喉科,南京210006 [3]南京医科大学神经生物学教研室江苏省人类功能基因组学重点实验室,南京211166

出　　处：《中华行为医学与脑科学杂志》2022年第7期577-582,共6页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：江苏省自然科学基金项目(BK20211008)。

摘　　要：目的利用静息态功能磁共振成像(functional magnetic resonance imaging,fMRI)技术探讨噪声性耳聋大鼠下丘和腹外侧眶皮层局部一致性与焦虑抑郁样行为的关系。方法 24只4周龄清洁级雄性SD大鼠按照随机数字表法分为噪声组和对照组,每组12只。噪声组大鼠置于宽带强噪声122 dB环境中2 h诱导双侧严重听力损失,对照组大鼠置于安静环境中。通过听觉脑干反应检测两组大鼠的听力情况,采用旷场实验评估大鼠的焦虑抑郁样行为,采用fMRI评估大鼠脑内局部一致性的差异。采用SPM12处理fMRI数据,采用SPSS 22.0软件通过Pearson相关分析计算fMRI数据与行为学的相关性。结果听觉脑干反应结果显示噪声组大鼠全频段听阈大于对照组大鼠[(85.417±6.463)dB,(20.083±8.853)dB,t=46.168,P<0.001],并在旷场实验中出现明显的焦虑抑郁样行为,即低活动水平。噪声组大鼠在旷场实验中的运动总距离[(39.912±5.696)m,(47.993±10.820)m,t=-2.289,P=0.032]、平均移动速度[(13.306±1.900)cm/s,(15.998±3.607)cm/s,t=-2.290,P=0.032]及站立次数[(13.333±5.960)次,(23.500±7.323)次,t=-3.730,P=0.001]均低于对照组。与对照组相比,噪声性耳聋大鼠下丘的局部一致性显著增强,而腹外侧眶皮层的局部一致性显著降低,且异常神经活动出现偏侧性。相关分析显示下丘的神经活动与噪声暴露大鼠在旷场中移动的总距离呈负相关(r=-0.691,P=0.013),而腹外侧眶皮层的神经活动与旷场焦虑抑郁样行为并未发现明显的相关性。结论噪声性耳聋大鼠大脑下丘神经活动与焦虑抑郁样行为密切相关,有助于阐明耳聋大鼠出现中枢障碍的神经病理学基础。Objective To investigate the local consistency of inferior colliculus and ventrolateral orbital cortex by resting-state functional magnetic resonance imaging(fMRI)in rats with noise induced deafness and its relationship with anxiety-and depression-like behavior.Methods Twenty-four clean grade male four-weeks old SD rats were randomly divided into noise group and control group with 12 rats in each group.Rats in the noise group were exposed to 122 dB broadband strong noise for 2 hours to induce severe bilateral hearing loss,while rats in the control group were placed in a quiet environment.Hearing thresholds were assessed by auditory brainstem response(ABR)test.The open field test(OFT)was conducted to examine anxiety-depression related behavior,and the local consistency in the rat brain was evaluated by fMRI.SPM12 software was used to process fMRI data,and Pearson correlation analysis was conducted by SPSS 22.0 software to calculate the correlation between fMRI data and behavior.Results The results of ABR showed that the full band hearing threshold of rats in the noise group was higher than that of rats in the control group((85.417±6.463)dB,(20.083±8.853)dB,t=46.168,P<0.001).And compared with control group,the rats in the noise group showed obvious anxiety-depression-like behavior in the open field test,that was,low activity level.The results of OFT showed that the total distance((39.912±5.696)m,(47.993±10.820)m,t=-2.289,P=0.032),average moving speed((13.306±1.900)cm/s,(15.998±3.607)cm/s,t=-2.290,P=0.032)and standing times((13.333±5.960),(23.500±7.323),t=-3.730,P=0.001)of the rats in the noise group were all lower than those in the control group.Compared with the control group,the local consistency of hypothalamus in the noise group was significantly enhanced,while the local consistency of ventrolateral orbital cortex was significantly reduced,and the abnormal neural activity was lateralized.The correlation analysis showed that the neural activity of the inferior colliculus was negatively correlated with th

关 键 词：噪声性耳聋 下丘 腹外侧眶皮层 焦虑 抑郁 大鼠 

分 类 号：R764.433[医药卫生—耳鼻咽喉科]