补肾益髓胶囊对实验性自身免疫性脑脊髓炎小鼠视网膜Müller及星形胶质细胞活化的影响  

Effects of Bushen Yisui Capsules on Muller cells and astrocyte activation in retina of experimental autoimmune encephalouyditis mice

作　　者：杨涛[1] 康越之 吴明慧 仝延萍 王昕[2] 王蕾[3] 樊永平[1] YANG Tao;KANG Yue-zhi;WU Ming-hui;TONG Yan-ping;WANG Xin;WANG Lei;FAN Yong-ping(Department of Traditional Chinese Medicine,Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,China;Beijing University of Chinese Medicine,Beijing 100029,China;School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学附属北京天坛医院中医科,北京100070 [2]北京中医药大学,北京100029 [3]首都医科大学中医药学院,北京100069

出　　处：《中华中医药杂志》2022年第7期4018-4021,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.82074350,No.81973599);北京市自然科学基金-北京市教育委员会科技计划重点项目(No.KZ/KM/SZ/SM201910025035);首都卫生发展科研专项(No.首发2020-2-2043)。

摘　　要：目的:观察补肾益髓胶囊对实验性自身免疫性脑脊髓炎(EAE)小鼠视网膜大胶质细胞(Müller细胞及星形胶质细胞)活化的影响,探讨补肾益髓胶囊对视网膜视神经节细胞(RGC)的保护作用机制。方法:将80只C57BL/6j小鼠随机分为正常组、模型组、芬戈莫德组与补肾益髓胶囊组,每组20只,皮下注射MOG35-55抗原乳剂诱导产生EAE。免疫当天,补肾益髓胶囊组予以3.02 g/kg灌胃,芬戈莫德组于免疫第15天给予0.3 mg/kg灌胃。于免疫第17天及35天取小鼠视网膜及眼球,免疫荧光观察EAE小鼠视网膜大胶质细胞活化及RGC凋亡情况,实时定量聚合酶链反应(qRT-PCR)检测EAE小鼠视网膜神经胶质纤维酸性蛋白(GFAP)及波形蛋白(Vimentin)基因的表达水平。结果:与模型组比较,补肾益髓胶囊能够显著减少EAE小鼠神经功能缺损(P<0.01),减少视网膜RGC细胞的凋亡(P<0.05,P<0.01);补肾益髓胶囊还能够显著缓解EAE小鼠第17、35天视网膜GFAP与Vimentin蛋白与基因的表达(P<0.05,P<0.01),减轻大胶质细胞活化(P<0.01)。结论:补肾益髓胶囊能够显著缓解EAE小鼠视网膜部位大胶质细胞的活化,减少视网膜RGC的凋亡,对EAE小鼠视网膜具有神经保护作用。Objective: To observe the effects of Bushen Yisui Capsules on the retinal macroglia(Müller cells and astrocytes)in mice with experimental autoimmune encephalomyelitis(EAE), and to reveal the protective mechanisms of Bushen Yisui Capsules on retinal ganglion cells(RGC). Methods: Eighty C57BL/6j mice were randomly divided into normal group, model group, Fingolimod group and Bushen Yishui Capsules group, 20 mice ineach group. C57BL/6j mice were injected with MOG35-55 antigen emulsion to induce EAE. The retina and eyeballs of EAE mice were taken on the day 17 and 35 postimmunization. The activation of retinal macroglia and apoptosis of RGC in EAE mice were observed by immunofluorescence. The expression levels of glial fibrillary acidic protein(GFAP) and Vimentin genes in EAE mice retina were detected by real-time quantitative polymerase chain reaction(qRT-PCR).Results: Compared with the model group, Bushen Yisui Capsules could significantly reduce the neurological deficit(P<0.01) and apoptosis of retinal RGC cells in EAE mice(P<0.05, P<0.01), Bushen Yisui Capsules could also significantly alleviate the expression of GFAP and Vimentin protein and gene in retina of EAE mice on the day 17 and 35 postimmunization(P<0.05, P<0.01), and reduce the activation of macroglia(P<0.01). Conclusion: Bushen Yisui Capsules could significantly alleviate the activation of macroglia in the retina of EAE mice and reduce the apoptosis of retinal RGC, and has a neuroprotective effect on the retina of EAE mice.

关 键 词：视神经炎 补肾益髓胶囊 星形胶质细胞 MÜLLER细胞 视神经节细胞 

分 类 号：R285.5[医药卫生—中药学]