微秒脉冲电场联合EPA对A-549细胞活性的影响  

Effects of Microsecond Pulsed Electric Field Combined with EPA on the Viability of A-549 Cells

作　　者：张建华[1] 杨延钧 吕彦鹏 金雯雯 陈传亮[2] ZHANG Jianhua;YANG Yanjun;LYU Yanpeng;JIN Wenwen;CHEN Chuanliang(School of Electrical Engineering,Zhengzhou University,Zhengzhou 450001,China;Department of Medical Imaging,Henan Provincial People's Hospital,Zhengzhou 450003,China)

机构地区：[1]郑州大学电气工程学院,河南郑州450001 [2]河南省人民医院放射科,河南郑州450003

出　　处：《郑州大学学报（工学版）》2022年第5期98-103,共6页Journal of Zhengzhou University（Engineering Science）

基　　金：河南省自然科学基金资助项目(202300410447);2020河南省高层次人才国际化资助项目。

摘　　要：针对当前电化学疗法中使用的传统化疗药物对人体正常组织毒副作用较大,中药成分对人体毒副作用小但作用效果弱等问题,选用中药单体成分二十碳五烯酸(EPA)作为抗癌药物,探究微秒脉冲电场对EPA细胞毒性效果的促进作用,以及两者联合作用对肺癌A-549细胞的毒性效果。首先,研究不同场强的脉冲电场作用下细胞膜通透性变化情况,以及不同的药物浓度(50~1 000μmol/L)、电场强度(750~2 000 V/cm)对A-549细胞活性的影响,采用PI染色法检测细胞膜通透性、CCK-8法检测细胞活性;其次,根据检测结果筛选用于后续实验的电场强度区间为1 000~1 500 V/cm, EPA浓度为100~400μmol/L;最后,设置电场联合药物组为实验组,空白对照组、纯药物组、纯电组为对照组,进行了脉冲电场联合EPA对A-549细胞毒性效果的实验研究,并且对脉冲电场联合EPA共同作用后培养24 h(48 h)时A-549细胞活性的变化情况进行了对比分析。研究表明:微秒脉冲电场能够显著增强EPA对A-549细胞的毒性效果,并且EPA浓度为300μmol/L时,脉冲电场和EPA的联合作用效果最佳。微秒脉冲电场对EPA细胞毒性的增强效果可达40%以上,为后续中药成分干预癌症的治疗提供了重要的促进手段。In view of the problems that traditional chemotherapeutic drugs used in current electrochemical therapy have large toxic and side effects on human normal tissues,and traditional Chinese medicine ingredients have little toxic and side effects on human body,but have weak effects.Eicosapentaenoic acid(EPA),a monomer component of traditional Chinese medicine,was selected as an anticancer drug to explore the promoting effect of microsecond pulse electric field on the cytotoxic effect of EPA,and the toxic effect of the combination of the two on lung cancer A-549 cells.Firstly,the permeability of cell membrane under the action of pulsed electric field with different electric field strengths,and the effects of different drug concentrations(50—1000μmol/L)and electric field strengths(750—2000 V/cm)on cell activity were studied.PI staining was used to detect cell membrane permeability,and CCK-8 assay was used to detect cell viability.Then,according to the detection results,the electric field intensity range for subsequent experiments was selected to be 1000—1500 V/cm,and the EPA concentration range was 100—400μmol/L.Finally,the electric field combined with the drug group was set as the experimental group,the blank control group,the pure drug group,and the pure electric group were set as the control group.The experimental study on the cytotoxicity of pulsed electric field combined with EPA on A-549 cells was carried out,and the changes of A-549 cell viability at 24 h(48 h)after the combined action of pulsed electric field and EPA were compared and analyzed.Research shows that microsecond pulsed electric field could significantly enhance the toxic effect of EPA on A-549 cells,and when the concentration of EPA was 300μmol/L,the combined effect of pulsed electric field and EPA was the best.The enhancement effect of microsecond pulsed electric field on EPA cytotoxicity could reach more than 40%,which provided an important promotion method for the subsequent intervention of traditional Chinese medicine ingredients in t

关 键 词：微秒脉冲电场 二十碳五烯酸 肺癌A-549细胞 细胞膜通透性 细胞活性 

分 类 号：N39[自然科学总论] TN782[电子电信—电路与系统]