新型牡蛎相关圆环病毒基因组的鉴定  

Identification of a novel oyster-related circovirus genome compa-rative genome analysis of oyster-related circoviruses

作　　者：杨李玲 郭迎香 位红颖 王萌 方艺菲 朱鹏 姜敬哲[2] YANG Liling;GUO Yingxiang;WEI Hongying;WANG Meng;FANG Yifei;ZHU Peng;JIANG Jingzhe(Fisheries College,Tianjin Agricultural University,Tianjin 300384,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China;Guangzhou Mega Gene Technology Co.,Ltd.,Guangzhou 510005,China;Bureau of Agriculture and Rural Affairs of Conghua District,Guangzhou 510300,China;Shanghai Meiji Biomedical Technology Co.,Ltd.,Shanghai 201321,China;Shanghai Ocean University,Shanghai 201318,China)

机构地区：[1]天津农学院,天津300384 [2]中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东广州510300 [3]广州美格基因科技有限公司,广东广州510005 [4]从化区农业农村局,广东广州510925 [5]上海美吉生物医药科技有限公司,上海201321 [6]上海海洋大学,上海201306

出　　处：《南方水产科学》2022年第4期65-75,共11页South China Fisheries Science

基　　金：国家自然科学基金面上项目(31972847);广东省省级现代农业产业技术体系“水产疫病监测与综合防控共性关键技术研发创新团队”(2019KJ141);中国水产科学研究院科技创新团队项目(2020TD42,2021SD05)。

摘　　要：病原基因组信息的匮乏是牡蛎病害研究进展缓慢的主要原因之一。为鉴定更多牡蛎相关病毒,基于前期研究获得的华南沿海多地养殖的香港牡蛎(Crassostrea hongkongensis)病毒组测序数据进行质控、组装、物种注释后,挑选出其中疑似圆环病毒基因组序列进行进化树构建、基因组比较、蛋白结构域分析、三维结构预测和病毒丰度分析,在养殖牡蛎体内鉴定到了5个序列完整的新型牡蛎相关圆环病毒基因组,且5个病毒基因组在多个样品中存在。结果显示,5条病毒序列与已知的圆环病毒聚类在一个大的分支,说明其为圆环病毒科成员;5条病毒基因组序列均含有一个复制酶蛋白基因,且均与节肢动物圆环病毒复制酶蛋白序列最为相似;5条序列与另外7条公共数据库检索到的序列形成了一个独立的子分支,且该分支的序列来源多为动物相关样品。基于结构域分析软件(SMART)鉴定到多数序列中存在复制酶的保守结构域。The lack of pathogen genome information is one of the main reasons for the slow progress of oyster disease research.In order to identify more oyster-associated viruses,based on the previously obtained viromic data of oysters(Crassostrea hongkongensis)cultured in multiple locations along the coast of the South China Sea,after quality control,assembly,and species annotation of the sequencing data,we selected the putative circovirus genome sequences for the construction of the phylogenet-ic tree,genome comparison,protein domain analysis,structure prediction and virus abundance analysis,which provides refer-ences for the research of oyster disease.The results show that five viral sequences clustered with known circoviruses in a large branch,indicating that they are members of the Circoviridae family.The five viral genome sequences all contained replication protein genes,with the highest similarity to a replication protein sequence of arthropod circovirus.The five sequences and seven other public database sequences formed an independent sub-branch.Viruses on this branch were mostly from animal-related samples.Based on the domain analysis(SMART),replicase conserved domains were identified in most sequences.

关 键 词：圆环病毒 生物信息分析 香港牡蛎 复制酶 结构域 

分 类 号：S917.4[农业科学—水产科学]