Distinctive Cassane Diterpenoids Corroborated Biogenetic Evolutionary Process from Caesalpinia mimosoides with Anti-renal Fibrosis Activity  

作　　者：Xinxin Zhang Yan Yin Yu Zhou Tianliang Zhu Miao Wang Huiyuan Gao 

机构地区：[1]School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang,Liaoning,110016 China [2]Key Laboratory of Structure-Based Drug Design&Discovery of Ministry of Education,Shenyang Pharmaceutical University,Shenyang,Liaoning,110016 China

出　　处：《Chinese Journal of Chemistry》2022年第5期617-627,共11页中国化学（英文版）

基　　金：The authors are grateful to the received financial support from the National Natural Science Foundation of China(No.31670359);the Liaoning Revitallzation Talents Program(No.XLYC 1905019);the Natural Science Foundation of Llaoning Province(No.201602691).

摘　　要：Eighteen diterpenoids were isolated from the seeds of Caesalpinia mimosoides,including four extremely infrequent cassane diterpenes,together with nine new and five known structures.Compounds 7 and 8 represent the first examples of cassane diterpenoids fused with a ketone carbonyl and a ternary oxygen ring at C-4,respectively.Compounds 9 and 10 possess a rare five-member ring in cassane diterpenes.Intriguingly,these isolated components are a clue to the fact that there are lower and higher levels in the plant secondary metabolites,demonstrating the evolution of secondary metabolism of C.mimosoides.All the isolated compounds were tested for their anti-renal fibrosis activity against TGF-β1 induced NRK-52E cell model.The mRNA levels of fibrosis markerα-SMA,E-cadherin,Collagen I were tested by qRT-PCR.The data showed compounds 6 and 9 exhibited the best anti-renal fibrosis activity and provided preliminary structure−activity relationships.

关 键 词：Cassane diterpene Caesalpinia mimosoides Natural products Molecular evolution Biological activity 

分 类 号：O62[理学—有机化学]