Genome-wide identification and characterization of basic helix-loop-helix transcription factors in Spodoptera litura upon pathogen infection  

作　　者：Jie Zhang Mengyao Zhu Qilin Li Ting Tang Liang Wen Jielai Zhong Ruonan Zhang Xiao-Qiang Yu Yuzhen Lu 

机构地区：[1]Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,Institute of Insect Science and Technology,School of Life Sciences,South China Normal University,Guangzhou,China [2]Guangzhou Key Laboratory of Insect Development Regulation and Application Research,Institute of Insect Science and Technology,School of Life Sciences,South China Normal University,Guangzhou,China

出　　处：《Insect Science》2022年第4期977-992,共16页昆虫科学（英文版）

基　　金：This research was funded by grants from the National Key R&D Program of China(No,2019YFD1002100);National Natural Science Foundation of China(No.31901941 and No.31970474);Natural Science Foun-dation of Guangdong Province(No.202102020966);China Postdoctoral Science Foundation(No.2020M682752).The authors are grateful for the generous gift of S.litura nucleopolyhedrovirus(SpItNPV)from Professor Kai Yang at Sun Yat-Sen University,Nomuruea rileyi(Nr06)from Professor Xusheng Liu at Central China Normal University,and Bacillus thuringiensis(WB9)from Professor Xiong Guan at Fujian Agriculture and Forestry University.

摘　　要：Basic helix-loop-helix(bHLH)transcription factors play an important role in a wide range of metabolic and developmental processes in eukaryotes,and bHLH proteins also participate in immune responses,especially in plants.However,their roles in insects upon entomopathogen infection are unknown.In this study,54 bHLH genes in 41 families were identified in a polyphagous pest,Spodoptera litura,including a new bHLH gene in group B,which is specifically present in Lepidoptera and was thus named Lep.The conserved amino acids in the bHLH domain,structural architecture,and chromosomal distribution of bHLH genes in S.litura were analyzed.The bHLH genes in Plutella xylostella and Apis mellifera were also updated,and genome-wide comparison and phylogenetic analysis of bHLH members in 5 holometabolous insects were performed.The expression profiles of S.litura bHLH(SlbHLH)genes in 3 tissues at different developmental stages and their responses to S.litura nucleopolyhedrovirus(SpltNPV),Nomuraea rileyi(Nr),and Bacillus thuringiensis(Bt)infection were investigated.More SlbHLHs in group B were expressed and differentially expressed during pathogen infections,and SlbHLHs tended to be downregulated in the midgut of S.litura larvae after B.thuringiensis treatment.Our study provides an overview of bHLH family members in S.litura and their responses to different pathogens used for pest biocontrol.These findings on bHLH members may contribute to uncovering the mechanism of host-pathogen interaction.

关 键 词：basic helix-loop-helix ENTOMOPATHOGEN INFECTION pest biocontrol Spodoptera liura 

分 类 号：Q96[生物学—昆虫学]