A fructose receptor gene influences development and feed intake in Helicoverpa armigera  

作　　者：Dong Ai Chenxi Dong Bin Yang Caihong Yu Guirong Wang 

机构地区：[1]School of Chemical and Environmental Engineering,China University of Mining and Technology(Beijing),Beijing,China [2]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing,China [3]Guangdong Laboratory of Lingnan Modern Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong Province,China

出　　处：《Insect Science》2022年第4期993-1005,共13页昆虫科学（英文版）

基　　金：This work was supported by National Natural Science Foundation of China(31725023,31861133019 to GW,and 31171912 to CY);Shenzhen Science and Technology Program(KQTD20180411143628272).

摘　　要：Gustatory receptors(GRs)are critical for multiple life activities of insects.Owing to the rapid development of genome and transcriptome sequencing,numerous insect GRs have been identified.However,the expression patterns and functions of these receptors are poorly understood.In this study,we analyzed the expression pattern of GRs in Helicoverpa armigera and found that the fructose receptor HarmGR9 was highly expressed in the foregut and abdomen.The function of HarmGR9 was identified using the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)system.Knockout of the HarmGR9 gene shortened the developmental period of the larval stages and increased food consumption in both larvae and adults.This study revealed the tissue distribution of sugar-sense-related receptors in H.armigera and thereby expanded the understanding of insect feeding regulation.

关 键 词：capillary feeding assay CRISPR/Cas9 FOREGUT gustatory receptor Helicoverpa armigeru 

分 类 号：S435.6[农业科学—农业昆虫与害虫防治]