Identification of two ABCC transporters involved in malathion detoxification in the red flour beetle,Tribolium castaneum  

作　　者：Janin Rösner Hans Merzendorfer 

机构地区：[1]Department of Chemistry-Biology,Institute of Biology,University of Siegen,Siegen,Germany

出　　处：《Insect Science》2022年第4期1096-1104,共9页昆虫科学（英文版）

基　　金：We are grateful to Johanne Tietmeyer for generating the used pGEM-T plasmids containing TcABC-cDNA inserts.This work was supported by a grant from the Deutsche Forschungsgemeinschaft(DFG Me2210/4-1).

摘　　要：ABC transporters have been suggested to be involved in insecticide detoxification in different insect species mainly based on the indirect observation of transcriptional upregulation of ABC gene expression in response to insecticide exposure.Previous studies performed by us and others in the red flour beetle,Tribolium castaneum,have analyzed the function of TcABCA-C and TcABCG-H genes using RNA interference(RNAi)and demonstrated that specific TcABCA and TcABCC genes are involved in the elimination of the pyrethroid tefluthrin and the benzoylurea diflubenzuron,because gene silencing increased the beetle's susceptibility to the insecticides.In this study,we focused on the potential functions of TcABCA-C genes in detoxification of the pyrethroid cyfluthrin(CF),the organophosphate malathion(MAL)and the diacylhdyazine tebufenozide(TBF).Analysis of transcript levels of selected TcABCA-C genes in response to treatment with these three chemically unrelated insecticides revealed that some genes were particularly upregulated after insecticide treatment.In addition,the ABC inhibitor verapamil synergized significantly the toxicity of MAL but only negligibly CF and TBF toxicities.Finally,silencing of two TcABCC genes by RNAi revealed a significant increase in susceptibility to MAL.In contrast,we did not observe a significant increase in insecticide-induced mortalities when knocking down TcABC genes in larvae treated with CF or TBF,although they were upregulated in response to insecticide treatment.Our results suggest that two pleiotropic ABCC transporters expressed in metabolic and excretory tissues contribute to the elimination of MAL.

关 键 词：ATP-binding assette transporters(ABC transporter) B-cy fluthrin insecti-cide deoxification MALATHION TEBUFENOZIDE Tribolium castaneum 

分 类 号：S432[农业科学—植物病理学]