Downregulation of NADPH-cytochrome P450 reductase via RNA interference increases the susceptibility of Acyrthosiphon pisum to desiccation and insecticides  

作　　者：Jian-Wen Qiao Yong-Liang Fan Bing-Jin Wu Tian-Tian Bai Ying-Hao Wang Zhan-Feng Zhang Dun Wang Tong-Xian Liu 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau,Ministry of Agriculture,Northwest A&F University,Yangling,Shaanxi Province,China [2]Key Lab of Integrated Crop Pest Management of Shandong Province,College of Plant Health and Medicine,Qingdao Agricultural University,Qingdao,Shandong Province,China

出　　处：《Insect Science》2022年第4期1105-1119,共15页昆虫科学（英文版）

基　　金：This research was supported by the National Natural Sci-ence Foundation of China(31772533);Shaanxi Key Project of Science and Tchnology(2020zdzx03-03-02).

摘　　要：Nicotinamide adenine dinucleotide phosphate(NADPH)-cytochrome P450 reductase(CPR)is involved in the metabolism of endogenous and exogenous substances,and detoxification of insecticides.RNA interference(RNAi)of CPR in certain insects causes developmental defects and enhanced susceptibility to insecticides.However,the CPR of Acyrthosiphon pisum has not been characterized,and its function is still not understood.In this study,we investigated the biochemical functions of A.pisum CPR(ApCPR).ApCPR was found to be transcribed in all developmental stages and was abundant in the embryo stage,and in the gut,head,and abdominal cuticle.After optimizing the dose and silencing duration of RNAi for downregulating ApCPR,we found that ApCPR suppression resulted in a significant decrease in the production of cuticular and internal hydrocarbon contents,and of cuticular waxy coatings.Deficiency in cuticular hydrocarbons(CHCs)decreased the survival rate of A.pisum under desiccation stress and increased its susceptibility to contact insecticides.Moreover,desiccation stress induced a significant increase in ApCPR mRNA levels.We further confirmed that ApCPR participates in CHC production.These results indicate that ApCPR modulates CHC production,desiccation tolerance,and insecticide susceptibility in A.pisum,and presents a novel target for pest control.

关 键 词：Acyrthosiphon pisum desiccation tolerance lhydrocarbon insecticide sus-ceptibility NADPH-cytochrome P450 reductase 

分 类 号：S43[农业科学—农业昆虫与害虫防治]