Discovery of a widespread presence bunyavirus that may have symbiont-like relationships with different species of aphids  

作　　者：Xin An Wei Zhang Chao Ye Guy Smagghe Jin-Jun Wang Jinzhi Niu 

机构地区：[1]Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing,China [2]International Joint Laboratory of China–Belgium on Sustainable Crop Pest Control,Academy of Agricultural Sciences,Southwest University,Chongqing,China [3]Department of Plants and Crops,Faculty of Bioscience Engineering,Ghent University,Ghent,Belgium

出　　处：《Insect Science》2022年第4期1120-1134,共15页昆虫科学（英文版）

基　　金：This research was supported by National Natural Sci-ence Foundation(32072423);National Natural Science Foundation of China-Major International(Regional)Joint Research Project(32020103010);111 Project(B18044);the Foundation Project of Southwest University(SWU019033).We thank Dr Bi-Yue Ding(Academy of Agricultural Sciences.Southwest Univer-sity)for the technical assistance in western bloting.The Ap-Uny colony and Ap-Efr colony were kindly pro-vided by Professors Tong-Xian Liu and Giuy Smagghe,respectively.

摘　　要：Aphids are important agricultural pests,vectors of many plant viruses and have sophisticated relationships with symbiotic microorganisms.Abundant asymptomatic RNA viruses have been reported in aphids due to the application of RNA-seq,but aphid-virus interactions remain unclear.Bunyavirales is the most abundant RNA virus order,which can infect mammals,arthropods,and plants.However,many bunyaviruses have specific hosts,such as insects.Here,we discovered 18 viruses from 10 aphid species by RNA-seq.Importantly,a widespread presence bunyavirus,Aphid bunyavirus 1(ABV-1),was determined to have a wide host range,infecting and replicating in all 10 tested aphid species.ABV-1 may be transmitted horizontally during feeding on plant leaves and vertically through reproduction.In a comparison of the physiological parameters of ABV-1high and ABV-1low strains of pea aphid,higher ABV-1 titers reduced the total nymphal duration and induced the reproduction.Moreover,viral titer significantly affected the lipid and protein contents in pea aphids.In summary,we proposed that ABV-1 may have stable symbiont-like relationships with aphids,and these observations may provide a new direction for studying bunyaviruses in aphids and establishing a model for virus-aphid interactions.

关 键 词：insec-specific virus pea aphid Phasmaviridae RNA virus RNAi 

分 类 号：S43[农业科学—农业昆虫与害虫防治]