一株蒲公英内生真菌Epicoccum sorghinum 1-2次级代谢产物研究  

Secondary metabolites from the dandelion-derived endophytic fungus Epicoccum sorghinum 1-2

作　　者：郝宝聪 郑瑶瑶 陈旭 陈秋霞 季若男 陈敏 HAO Bao-cong;ZHENG Yao-yao;CHEN Xu;CHEN Qiu-xia;JI Ruo-nan;CHEN Min(Marine Science&Technology Institute,College of Environmental Science&Engineering,Yangzhou University,Yangzhou 225127,China;Key Laboratory of Marine Drugs,the Ministry of Education of China,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China)

机构地区：[1]扬州大学环境科学与工程学院,海洋科学与技术研究所,江苏扬州225127 [2]中国海洋大学医药学院,海洋药物教育部重点实验室,山东青岛266003

出　　处：《药学学报》2022年第7期2115-2119,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81703411);大学生创新创业训练计划项目资助(X20180476)。

摘　　要：运用硅胶柱、凝胶柱和半制备高效液相色谱等方法对一株蒲公英内生真菌Epicoccum sorghinum 1-2发酵产物中的次级代谢产物进行分离纯化,通过核磁共振谱(NMR)及质谱(MS)等现代波谱分析方法进行化合物的结构解析,并采用滤纸片法测定化合物的抗菌活性。最终从E.sorghinum 1-2的发酵产物中分离鉴定了7个化合物,分别为(4R*,5R*,6S*)-4,5-二羟基-6-(6'-甲基水杨酸氧基)-2-甲氧基甲基-2-环己烯-1-酮(1)、(4R*,5R*,6S*)-4,5-二羟基6-(6′-甲基水杨酸氧基)-2-甲基-2-环己烯-1-酮(2)、(4R,5R,6S)-4,5-二羟基-6-(6′-甲基水杨酸氧基)-2-羟基甲基-2-环己烯-1-酮(3)、(-)-gabosine E(4)、theobroxide(5)、3-氯代龙胆醇(6)和3-羟基苯甲醇(7)。其中化合物1~5是顶环氧菌素类化合物,化合物6和7是苯酚类化合物。化合物1和2为首次报道的新化合物,化合物6对金葡菌具有显著的抑菌作用。The secondary metabolites from the dandelion-derived Epicoccum sorghinum 1-2 were isolated by silica gel and Sephadex gel column chromatography,and semi-preparative high performance liquid chromatography(HPLC).Their structures were identified by comprehensive NMR and MS methods.Their antibacterial activities were determined by filter paper method.Finally,seven compounds were isolated and identified from the fermenta‐tion product of E.sorghinum 1-2,including(4R*,5R*,6S*)-4,5-dihydroxy-6-(6'-methylsalicyloxy)-2-methoxymethyl2-cyclohexen-l-one(1),(4R*,5R*,6S*)-4,5-dihydroxy-6-(6′-methylsalicyloxy)-2-methyl-2-cyclohexen-1-one(2),(4R,5R,6S)-4,5-dihydroxy-6-(6'-methylsalicyloxy)-2-hydroxymethyl-2-cyclohexen-1-one(3),(-)-gabosine E(4),theobroxide(5),3-chlorogentisyl alcohol(6),and 3-hydroxybenzyl alcohol(7),of which 1-5 are epoxydons,and 6 and 7 are phenolics.Compounds 1 and 2 are new structures reported for the first time.Compound 6 showed significant antibacterial activity against Staphylococcus aureus.

关 键 词：蒲公英内生真菌 Epicoccum sorghinum 次级代谢产物 顶环氧菌素 抗菌活性 

分 类 号：R284[医药卫生—中药学]