岭南草药九节总黄酮提取和富集工艺研究  

Optimization of Extraction and Enrichment Technologies of Total Flavonoids in Psychotria asiatica from Lingnan

作　　者：尹丽 卢成淑 王华坤 李国利 许婷婷 YIN Li;LU Chengshu;WANG Huakun;LI Guoli;XU Tingting(Yulin Normal University,Yulin 537000,China;Bioengineering&Technology Center for Native Medicinal Resources Development,Yulin Normal University,Yulin 537000,China)

机构地区：[1]玉林师范学院,玉林537000 [2]玉林师范学院地产药用资源开发与生物工程技术中心,玉林537000

出　　处：《世界中医药》2022年第13期1883-1889,共7页World Chinese Medicine

基　　金：国家自然科学基金项目(81960658)——基于iTRAQ蛋白质组学和网络药理学技术的九节抗抑郁机制研究;广西教育厅中青年教师科研基础能力提升项目(2021KY0584)——大鼠海马组织TMT蛋白质组学分析;玉林师范学院高层次人才科研启动项目(G2018027)——九节木抗抑郁活性研究。

摘　　要：目的:对采用响应面法优化九节中总黄酮的提取工艺后以大孔树脂富集的工艺进行评价。方法:在单因素实验基础上,以九节总黄酮提取率作为响应值,以乙醇浓度、提取温度、料液比和提取时间为自变量,采用Box-Behnken响应面法,根据其中心组合的实验原理优化工艺条件,再以吸附、解吸及回收效果等为指标筛选最优的大孔树脂,对总黄酮进一步富集。结果:最优提取工艺为:提取温度59℃,乙醇浓度75%,料液比1∶10,提取时间48 min,提取次数为2次,在此条件下浸膏得率25.60%,总黄酮得率为2.36%,HP-20型树脂对九节总黄酮有较好的富集效果,富集后总黄酮回收率达到87.70%,纯度相比浸膏提升6倍。结论:该工艺简单且稳定性好,对于九节作为药品资源的开发利用具有重要的参考价值。Objective:To optimize the extraction process of total flavonoids from Psychotria asiatica L.using response surface methodology followed by evaluation with enrichment technology by macroporous adsorption resin.Methods:On the basis of single factor experiment,with the ethanol concentration,extraction temperature,solid-liquid ratio,and extraction time as independent variables and the yield of total flavonoids as the response value,the process conditions were optimized by the Box-Behnken experiment.Moreover,the optimum macroporous resin was screened out in the enrichment process using adsorption,desorption,and recovery effect as indexes.Results:The results showed that the optimal extraction conditions were as follows:extraction temperature of 59℃,ethanol concentration of 75%,solid-liquid ratio of 1∶10,extraction time of 48 min,and extraction twice.Under the optimized conditions,the extraction rate was 25.60% and the yield of total flavonoids was 2.36%.The HP-20 resin showed a good enrichment effect on total flavonoids of P.asiatica.After enrichment,the recovery rate of total flavonoids reached 87.70%,and the purity of total flavonoids was 6 times higher than that of the crude extract.Conclusion:The optimized process is simple and stable and can provide references for the development and utilization of P.asiatica L.

关 键 词：岭南草药 九节 总黄酮 提取 富集 响应面法 最佳提取工艺 大孔树脂 

分 类 号：R282[医药卫生—中药学]