基于慢性轻度不可预知性温和型应激的C57BL/6N小鼠抑郁模型的建立与评价  

Establishment and Evaluation of C57BL/6N Mice with Chronic Unpredictable Mild Stress-induced Depression

作　　者：约日古丽·麦海提 任小巧[1] 范佳佳[1] 李鑫 刘媛媛 毛萌[1] Yuriguli·MAIHAITI;REN Xiaoqiao;FAN Jiajia;LI Xin;LIU Yuanyuan;MAO Meng(Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《世界中医药》2022年第13期1895-1901,共7页World Chinese Medicine

基　　金：青海省科技厅重点研发计划项目(2021-SF-C21)。

摘　　要：目的:探索慢性轻度不可预知性温和型应激(CUMS)法建立抑郁症C57BL/6N小鼠模型的条件,观察不同时点该模型行为学变化,对该模型进行评价和优化。方法:对C57BL/6N小鼠进行连续8周的CUMS应激来诱导其抑郁状态。分别在造模3、5、8周时通过糖水偏好实验(SPT)、旷场实验(OFT)、强迫游泳实验(FST)、悬尾实验(TST)等行为学实验,观察小鼠抑郁样行为的改变。结果:与对照组相比,发现各时点CUMS组小鼠一般状态均较差,出现毛发脱落、体型瘦小、活动减少等;造模3、5、8周时均出现糖水偏好率下降,FST和TST不动时间的延长,OFT垂直运动次数减少等,提示CUMS组小鼠出现快感缺失、绝望感及探索能力下降;造模8周时出现OFT水平运动总路程缩短、运动速度减慢,提示CUMS组小鼠出现了自发运动能力下降。结论:CUMS应激可以成功建立C57BL/6N小鼠抑郁模型,并且随着应激时间的延长,C57BL/6N小鼠的抑郁表现逐渐加重。Objective:To explore the conditions for inducing depression in C57BL/6N mice by chronic unpredictable mild stress(CUMS),observe the behavioral changes of the model mice at different time points,and evaluate and optimize the model.Methods:Depression was induced in C57BL/6N mice by CUMS(8 weeks).The changes of depression-like behaviors of mice were observed based on the sucrose preference test(SPT),open field test(OFT),forced swim test(FST),and tail suspension test(TST)at the 3rd,5th,and 8th weeks of modeling.Results:Compared with the control group,mice in the CUMS group showed poor conditions at each time point,as manifested by the hair loss,emaciation,reduced activity,etc.At the 3rd,5th,and 8th weeks,the model group demonstrated low sucrose preference percentage,long immobility time in FST and TST,and the few vertical movements in OFT,suggesting anhedonia,despair,and decreased ability of exploration.After 8 weeks of modeling,the total horizontal distance traveled decreased and the movement became slow in OFT in the model group,an indicative of decreased spontaneous motor ability of mice in CUMS group.Conclusion:CUMS is applicable to the depression modeling with C57BL/6N mice,and the depression aggravates gradually over time.

关 键 词：抑郁症 CUMS应激 C57BL/6N小鼠 行为学 

分 类 号：R277.7[医药卫生—中医学]