玉米TRIBOA-Glc邻甲基转移酶基因bx7的克隆及其生物信息学分析  

Cloning and bioinformatic analysis of maize TRIBOA-Glc O-methyltransferase gene bx7

作　　者：徐碧莹 黄伟鹏 张敏艳 吴委林[1] 郑大浩[1] XU Biying;HUANG Weipeng;ZHANG Minyan;WU Weilin;ZHENG Dahao(Agricultural College of Yanbian University,Yanji Jilin 133002,China)

机构地区：[1]延边大学农学院,吉林延吉133002

出　　处：《延边大学农学学报》2022年第2期21-28,共8页Agricultural Science Journal of Yanbian University

基　　金：国家自然科学基金项目(31660395);吉林省科技厅基础研究专项(202002026JC)。

摘　　要：苯并噁嗪类次生代谢物与植株防御响应密切相关。TRIBOA-Glc邻甲基转移酶是苯并噁嗪代谢中的关键酶之一。为了解编码TRIBOA-Glc邻甲基转移酶的bx7基因,该试验采用cDNA-PCR克隆技术,从高抗蚜虫的Mo17中克隆得到bx7基因。其生物信息学分析表明:从Mo17中分离的目标序列长度为1522 bp,具有完整的开放阅读框(ORF),与标准基因序列比较,存在16处突变,编码氨基酸数相差1,分别为392、391,氨基酸序列存在6处区别,导致蛋白质二级结构和三级结构差异显著,分子量分别为42.52 KD、42.53 KD,等电点分别为5.47、5.41;通过对氨基酸序列的分析发现,该蛋白质为无跨膜结构的亲水性蛋白,进化分析结果显示与谷子和短柄草的亲缘关系最近,并且该蛋白的保守结构域属于dimerization2超级家族和AdoMet_MTases超级家族。The secondary metabolites of benzoxazines are closely related to the defense response of plants.TRIBOA-Glc O-methyl transferase is one of the key enzymes in benoxazine metabolism.This study cloned bx7 gene encoding TRIBOA-Glc O-methyltransferase from Mo17 of high resistance to aphid by cDNA-PCR bx7 and implemented the bioinformatics analysis.The result showed that the length of bx7 sequence was 1522 bp with a complete open reading frame.Compared with the standard gene sequence,the cloned bx7 had 16 mutations,the number of the coding amino acids was 392(the standard gene was 391),and had 6 differences in amino acid sequences,resulting in the significant differences of protein secondary and tertiary structure,which the molecular weights of both structure proteins were 42.52 KD and 42.53 KD,respectively,and the isoelectric points were 5.47 and 5.41,respectively.Furthermore,the protein was a hydrophilic protein without transmembrane structure.The result of the evolutionary analysis showed that bx7 from Mo17 was closed to millet and brachygrass and its conserved domain belonged to the Dimerization2 super family and AdoMet_MTases super family.

关 键 词：玉米 基因克隆 TRIBOA-Glc邻甲基转移酶 生物信息学分析 

分 类 号：S513[农业科学—作物学]