功能菌群DDMZ1共代谢脱色降解不同结构染料差异效应  

Differential effect of DDMZ1 cometabolic decolorization on the degradation of dyes with different structures

作　　者：范娇 谢学辉 秦艳 陈小光 方英荣 杨珊珊 赵江贵 莫浩楠 郑航蜜 刘娜 张庆云 宋新山 FAN Jiao;XIE Xuehui;QIN Yan;CHEN Xiaoguang;FANG Yingrong;YANG Shanshan;ZHAO Jianggui;MO Haonan;ZHENG Hangmi;LIU Na;ZHANG Qingyun;SONG Xinshan(School of Environmental Science and Engineering,Donghua University,National Environmental Protection Textile Industry Pollution Control Engineering Technology Center,China National Textile Industry Federation Key Laboratory of Textile Pollution Control and Emission Reduction Technology,Shanghai 201620,China;Shanghai Institute of Pollution Control and Ecological Security,Shanghai 200092,China;School of Environment and Surveying Engineering,Suzhou University,Suzhou 234000,Anhui,China;School of Chemistry and Chemical Engineering Anhui University of Engineering Technology,Wuhu 241000,Anhui,China)

机构地区：[1]东华大学环境科学与工程学院,国家环境保护纺织工业污染防治工程技术中心,中国纺织工业联合会纺织行业污染治理与减排技术重点实验室,上海201620 [2]上海污染控制与生态安全研究院,上海200092 [3]宿州学院环境与测绘工程学院,安徽宿州234000 [4]安徽工程大学化学与环境工程学院,安徽芜湖241000

出　　处：《化工进展》2022年第8期4449-4463,共15页Chemical Industry and Engineering Progress

基　　金：国家重点研究发展计划(2019YFC0408304);中央高校基本科研业务费专项资金(2232019D3-22);上海市自然科学基金面上项目(21ZR1402100);安徽省自然科学基金(1808085QE176);宿州学院优秀学术技术骨干项目(2020XJGG06);安徽工程大学引进人才科研启动基金(2020YQQ059);安徽工程大学校级科研项目(Xjky2020086)。

摘　　要：为探究功能菌群DDMZ1在相同共基质条件下脱色降解五类不同结构染料的效能差异情况及微观生物学机制,本文首先测定了功能菌群DDMZ1在酵母提取物(yeast extract,YE)共基质条件下对五类不同结构共12种染料的脱色率,其次通过液相色谱-飞行时间串联质谱联用仪(LC-TOF-MS)进一步鉴定了其中5种典型的不同结构染料在YE共基质体系中的降解产物,推导了各自的降解途径,最后采用Illumina Miseq高通量测序评估了YE共基质条件下由5种典型的不同结构染料引起的微生物群落结构差异以及优势功能菌属的富集情况,进而揭示了YE共基质条件下染料结构、群落结构、优势菌属与染料脱色性能之间的相互作用关系。脱色结果表明:添加YE共基质能够显著促进菌群脱色性能,对浓度为100mg/L的12种不同结构的染料作用72h后,偶氮类染料脱色率均可达到80%以上,三苯甲烷类染料尤其是孔雀石绿由几乎为0的脱色率提升到了98.3%,蒽醌类染料的脱色率也均提升了20%左右。LC-TOF-MS分析产物表明5种典型的不同结构染料在YE共基质作用下均被生物降解成分子量小且结构简单的代谢产物。高通量测序结果表明,相较于无机物培养基条件,共基质YE的添加能显著增加各种不同结构染料样品中菌群群落结构的多样性。而在相同共基质YE条件下,不同结构染料样品中的优势功能菌属明显不同。如在偶氮类染料样品YEAO7、YERB5、YECBE中,最优势功能菌属为志贺氏菌属(占比依次为32.33%、38.41%、33.96%),在蒽醌类染料样品YERBBR中最优势功能菌属为寡氧单胞菌属(47.40%),而在三苯甲烷类染料样品YEMG中假单胞菌属(92.79%)为绝对优势功能菌属。以上结果表明,类似结构的染料可能富集相似的优势功能菌属,而不同结构的染料则明显富集不同的优势功能菌属。In order to explore the difference of decolorization efficiency and microbiological mechanism of functional flora DDMZ1 on five different dyes under the same co-matrix condition,the decolorization rate of functional bacteria DDMZ1 on 12 different dyes with five distinct structures was measured under the co-matrix condition of Yeast Extract(YE).Then,the degradation products of five typical dyes with different structures in YE co-matrix system were further identified by liquid chromatography/time-of-flight/mass spectrometry(LC-TOF-MS),and their degradation pathways were deduced.Finally,Illumina Miseq sequencing was utilized to evaluate the distinction in microbial community structure and the enrichment of dominant functional bacteria in YE co-substrate caused by five typical dyes with different structures,and further revealed the interactions between dye structures,community structures,dominant bacteria,and decolorization performances of dyes in YE co-metabolism substrate.The decolorization results indicated that,the addition of YE co-matrix could promote the decolorization efficiency of bacterial community significantly.After 72h treatment with 12 different structures of dyes at the concentration of 100mg/L,the decolorization rate of azo dyes could reach more than 80%,and the decolorization rate of triphenylmethane dyes,especially malachite green,increased from almost 0 to 98.3%.The decolorization rate of anthraquinone dyes also increased by about 20%.LC-TOF-MS analysis showed that the five typical dyes with different structures were biodegraded into metabolites with small molecular weight and simple structures under the action of YE co-substrate.The findings of high-throughput sequencing manifested that the addition of co-matrix YE notably increased the diversity of bacterial community structures in different dye samples compared with the mineral nutrients(MN)medium.Under the same co-matrix condition of YE,the dominant functional bacteria of dye samples with different structures were obviously different from each

关 键 词：印染废水 脱色菌群 共代谢 染料结构 

分 类 号：X788[环境科学与工程—环境工程]