The RALF1-FERONIA complex interacts with and activates TOR signaling in response to low nutrients  

作　　者：Limei Song Guoyun Xu Tingting Li Huina Zhou Qinlu Lin Jia Chen Long Wang Dousheng Wu Xiaoxu Li Lifeng Wang Sirui Zhu Feng Yu 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Biology,and Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation,Hunan University,Changsha 410082,P.R.China [2]Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,P.R.China [3]National Engineering Laboratory for Rice and By-product Deep Processing,Central South University of Forestry and Technology,Changsha 410004,P.R.China [4]Technology Center,China Tobacco Hunan Industrial Co.,Ltd.,Changsha 410007,P.R.China [5]State Key Laboratory of Hybrid Rice,Hunan Agricultural Biotechnology Research Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,P.R.China

出　　处：《Molecular Plant》2022年第7期1120-1136,共17页分子植物（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(NSFC-;31900232,31871396,and 31571444);the Natural Science Foundation of Hunan Province(2020JJ5049);the Science and Technology Innovation Program of Hunan Province(2020WK2014,and 2022WK2007);the Key Scientific and Technological Project of Henan Province(212102110446);the China Postdoctoral Science Foundation(2018M642972).

摘　　要：Target of rapamycin(TOR)kinase is an evolutionarily conserved major regulator of nutrient metabolism and organismal growth in eukaryotes.In plants,nutrients are remobilized and reallocated between shoots and roots under low-nutrient conditions,and nitrogen and nitrogen-related nutrients(e.g.,amino acids)are key upstream signals leading to TOR activation in shoots under low-nutrient conditions.However,how these forms of nitrogen can be sensed to activate TOR in plants is still poorly understood.Here we report that the Arabidopsis receptor kinase FERON IA(FER)interacts with the TOR pathway to regulate nutrient(nitrogen and amino acid)signaling under low-nutrient conditions and exerts similar metabolic effects in response to nitrogen deficiency.We found that FER and its partner,RPM1-induced protein kinase(RIPK),interact with the TOR/RAPTOR complex to positively modulate TOR signaling activity.During this process,the receptor complex FER/RIPK phosphorylates the TOR complex component RAPTOR1B.The RALF1 peptide,a ligand of the FER/RIPK receptor complex,increases TOR activation in the young leaf by enhancing FER-TOR interactions,leading to promotion of true leaf growth in Arabidopsis under lownutrient conditions.Furthermore,we showed that specific amino acids(e.g.,Gin,Asp,and Gly)promote true leaf growth under nitrogen-deficient conditions via the FER-TOR axis.Collectively,our study reveals a mechanism by which the RALF1-FER pathway activates TOR in the plant adaptive response to low nutrients and suggests that plants prioritize nutritional stress response over RALF1-mediated inhibition of cell growth under low-nutrient conditions.

关 键 词：TOR FERON IA nitrogen response amino acid response energy metabolism 

分 类 号：Q943.2[生物学—植物学]