Genome architecture and tetrasomic inheritance of autotetraploid potato  

作　　者：Zhigui Bao Canhui Li Guangcun Li Pei Wang Zhen Peng Lin Cheng Hongbo Li Zhiyang Zhang Yuying Li Wu Huang Mingwang Ye Daofeng Dong Zhukuan Cheng Peter VanderZaag Evert Jacobsen Christian W.B.Bachem Suomeng Dong Chunzhi Zhang Sanwen Huang Qian Zhou 

机构地区：[1]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China [2]Key Laboratory for Potato Biology of Yunnan Province,The CAAS-YNNU-YINMORE Joint Academy of Potato Science,Yunnan Normal University,Kunming 650500,China [3]Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop,Ministry of Agriculture and Rural Affairs,Beijing,100081.,China [4]Plant Breeding,Wageningen University and Research,Droevendaalsesteeg 1,6708 PB Wageningen,the Netherlands [5]Vegetable Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China [6]State Key Lab of Plant Genomics,Institute of Genetics and Developmental Biology,Innovation Academy for Seed Design,Chinese Academy of Sciences,Beijing 100101,China [7]Sunrise Potato Storage Ltd.,Alliston,ON L9R1V3,Canada [8]Department of Plant Pathology and Key Laboratory of Plant Immunity,Nanjing Agricultural University,Nanjing 210095,China [9]Peng Cheng Laboratory,Shenzhen 518055,China

出　　处：《Molecular Plant》2022年第7期1211-1226,共16页分子植物（英文版）

基　　金：supported by the Guangdong Major Project of Basic and Applied Basic Research(2021B0301030004);Agricultural Science and Technology Innovation Program(CAAS-ZDRW202101);to S.H.This work was also supported by the National Natural Science Foundation of China(grant nos.31561143006 to G.L.and 32001601 to Y.L.).

摘　　要：Potato(Solanum tuberosum)is the most consumed non-cereal food crop.Most commercial potato cultivars are autotetraploids with highly heterozygous genomes,severely hampering genetic analyses and improvement.By leveraging the state-of-the-art sequencing technologies and polyploid graph binning,we achieved a chromosome-scale,haplotype-resolved genome assembly of a cultivated potato,Cooperation-88(C88).lntra-haplotype comparative analyses revealed extensive sequence and expression differences in this tetraploid genome.We identified haplotype-specific pericentromeres on chromosomes,suggesting a distinct evolutionary trajectory of potato homologous centromeres.Furthermore,we detected double reduction events that are unevenly distributed on haplotypes in 1021 of 1034 selfing progeny,a feature of autopolyploid inheritance.By distinguishing maternal and paternal haplotype sets in C88,we simulated the origin of heterosis in cultivated tetraploid with a survey of 3110 tetra-allelic loci with deleterious mutations,which were masked in the heterozygous condition bytwo parents.This study provides insights into the genomic architecture of autopolyploids and will guide their breeding.

关 键 词：potato genome polyploid genome haplotype-resolved assembly haplotype diversity potato centromere 

分 类 号：S532[农业科学—作物学]