基于显微和傅里叶红外光谱法鉴别不同基原品种的白族药肺心草  

Study on Different Primordial Varieties of Bai Medicine Delavay Parnassia Herb Based on Microstructure and FTIR

作　　者：闻焜 代欣桃 段宝忠 Wen Kun;Dai Xintao;Duan Baozhong(School of Pharmacy,Dali University,Yunan Dali 671000,China;Dali Institute for Food and Drug Control)

机构地区：[1]大理大学药学院,云南大理671000 [2]大理州食品药品检验所

出　　处：《中国药师》2022年第8期1448-1451,1480,共5页China Pharmacist

基　　金：云南省重大科技专项(编号:202002AA100007)。

摘　　要：目的:基于显微性状和化学成分特征,探讨白族药肺心草两种基原药材混用的合理性。方法:收集不同产地的39批肺心草药材,使用光学显微镜,观察两种基原药材的根、根茎、叶的组织结构以及粉末特征。采用傅里叶红外光谱(FTIR)结合系统聚类分析(HCA)及主成分分析(PCA),对两种药材的整体化学成分进行研究,并将红外鉴别、经验鉴别及显微鉴别结果相互印证。结果:不同基原的肺心草显微特征区别明显;红外光谱结果HCA、PCA分析结果显示,阴地蕨和绒毛阴地蕨明显区分,表明化学成分含量差异明显。结论:本试验采用显微和傅里叶红外光谱两种方法均能鉴别两种基原品种,与经验鉴别相比一致性较好,可为肺心草药材整体质量评价提供一定的参考依据。Objective: To explore the mixing rationality of two primordial varieties of Bai medicine delavay parnassia herb based on the microscopic traits and chemical composition characteristics. Methods: Totally 39 batches of delavay parnassia herb from different origins were collected. The tissue structure and powder characteristics of roots, rhizomes and leaves of them were observed by conventional microscopy. Fourier transforms infrared spectroscopy(FTIR) combined with pattern recognition methods including hierarchical cluster analysis(HCA) and principal component analysis(PCA) was helpful to investigate the difference in comprehensive chemical compositions. Finally, the results of IR, empirical identification and microscopic identification were mutually verified. Results: The microscopic characteristics of different primordial varieties of delavay parnassia herb were obviously different;FTIR combined with HCA and PCA showed that the chemical ingredients of two medicinal herbs were significantly different. Conclusion: The two methods of microscopy and Fourier transform infrared spectroscopy can be used to identify the two original varieties of delavay parnassia herb, which are in good agreement with the empirical identification results. The results can provide certain reference for the overall quality evaluation of delavay parnassia herb.

关 键 词：肺心草 显微特征 傅里叶红外光谱 主成分分析 聚类分析 

分 类 号：R282.5[医药卫生—中药学]