北京市PM_(2.5)中类腐殖质的生物氧化应激效应初探  

Preliminary Study on Biological Oxidative Stress Effects of Humic-Like Substances in PM_(2.5) in Beijing

作　　者：张东 张元勋 尚晶 薛凯兵 马健 陈琦[5] ZHANG Dong;ZHANG Yuanxun;SHANG Jing;XUE Kaibing;MA Jian;CHEN Qi(College of Resources and Environment,University of Chinese Academy of Sciences,Beijing 100049,China;Beijing Yanshan Earth Critical Zone National Research Station,University of Chinese Academy of Sciences,Beijing 101408,China;Institute of Eco-Environmental Forensics,Shandong University,Qingdao 266237,China;Institute of Urban Meteorology,China Meteorological Administration,Beijing 100089,China;College of Environmental Sciences and Engineering,Peking University,Beijing 100871,China)

机构地区：[1]中国科学院大学资源与环境学院,北京100049 [2]北京燕山地球关键带国家野外科学观测研究站,北京101408 [3]山东大学生态环境损害鉴定研究院,山东青岛266237 [4]北京城市气象研究院,北京100089 [5]北京大学环境科学与工程学院,北京100871

出　　处：《环境科学研究》2022年第8期1986-1995,共10页Research of Environmental Sciences

基　　金：国家自然科学基金项目(No.41877310)。

摘　　要：为研究细颗粒物(PM_(2.5))中类腐殖质(humic-like substances,HULIS)的生物氧化应激效应,对北京市区和郊区不同季节大气PM_(2.5)及其中碳质组分和金属元素浓度进行测定,并以大鼠肺泡巨噬细胞(NR8383)作为细胞模型,分析PM_(2.5)及其重要组分诱导产生内源性ROS的能力,研究了PM_(2.5)中HULIS对诱导产生内源性ROS的贡献.结果表明:①冬季PM_(2.5)中碳质组分浓度及内源性ROS浓度(单位为μg zymosan/m^(3),以酵母聚糖(zymosan)表征的单位体积空气中内源性ROS的浓度)明显高于夏季,但夏季PM_(2.5)具有更高的生物氧化潜力(单位为μg zymosan/mg PM,以酵母聚糖(zymosan)表征的单位质量颗粒物的生物氧化潜力).②PM_(2.5)中碳质组分和一些金属元素浓度均与PM_(2.5)生成的内源性ROS浓度之间具有较高的相关性.③HULIS单独诱导生成的内源性ROS浓度也呈冬季〔市区和郊区分别为(55.46±22.69)(43.27±23.89)μg zymosan/m^(3)〕高于夏季〔市区和郊区分别为(18.73±11.94)(7.88±3.63)μg zymosan/m^(3)〕的特征.在北京市区和郊区两个采样点,由HULIS单独诱导生成的内源性ROS浓度与PM_(2.5)诱导生成的总内源性ROS浓度之间具有较好的相关性.研究显示,HULIS是PM_(2.5)中诱导ROS生成的关键组分,在北京市区和郊区两个采样点HULIS对PM_(2.5)诱导的内源性ROS的贡献率分别为42%和37%.In order to study the biological oxidative stress effect of humic-like substances(HULIS)in fine particulate matter(PM_(2.5)),the concentrations of carbonaceous components and metal elements in atmospheric PM_(2.5) in the urban and suburb areas of Beijing were measured.Rat alveolar macrophages(NR8383)were used as cell model to determinate the ability of PM_(2.5) and its important components to induce endogenous ROS and the contribution of HULIS in PM_(2.5) to the induction of endogenous ROS.The results showed that:(1)The concentrations of carbonaceous components and endogenous ROS(the unit isμg zymosan/m^(3),representing the concentration of endogenous ROS per unit volume of air)in PM_(2.5) in winter were significantly higher than those in summer,but PM_(2.5) in summer had higher biological oxidative potential(the unit isμg zymosan/mg PM,representing the biooxidative potential of the unit mass of particulate matter).(2)The concentrations of carbonaceous components and some metal elements in PM_(2.5) all had high correlation with the concentration of endogenous ROS induced by fine particulate matter.(3)The endogenous ROS induced by HULIS alone was also higher in winter((55.46±22.69)(43.27±23.89)μg zymosan/m^(3) in urban and suburbs,respectively)than in summer((18.73±11.94)(7.88±3.63)μg zymosan/m^(3) in urban and suburbs,respectively).There was also a good correlation between the endogenous ROS concentration induced by HULIS alone and the total endogenous ROS concentration induced by PM_(2.5) at the two sampling sites in the urban and suburban areas of Beijing.The results showed that HULIS was a key component that induced ROS generation in PM_(2.5) at the two sampling sites in the urban and suburban areas of Beijing,and the contribution rates of HULIS to PM_(2.5)-induced endogenous ROS were 42%and 37%,respectively.

关 键 词：PM_(2.5) 类腐殖质(HULIS) 活性氧(ROS) 生物氧化应激 

分 类 号：X51[环境科学与工程—环境工程]