Magnetic-activated cell sorting of nonapoptotic spermatozoa with a high DNA fragmentation index improves the live birth rate and decreases transfer cycles of IVF/ICSI  

作　　者：Jie Mei Lin-Jun Chen Xin-Xin Zhu Wen Yu Qing-Qiang Gao Hai-Xiang Sun Li-Jun Ding Jun-Xia Wang 

机构地区：[1]Center for Reproductive Medicine,Department of Obstetrics and Gynecology,Nanjing Drum Tower Hospital,The Affiliated Hospital of Nanjing University Medical School,Nanjing 210008,China [2]Center for Molecular Reproductive Medicine,Nanjing University,Nanjing 210008,China [3]Department of Andrology,Nanjing Drum Tower Hospital,The Affiliated Hospital of Nanjing University Medical School,Nanjing 210008,China [4]Clinical Center for Stem Cell Research,The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing 210008,China

出　　处：《Asian Journal of Andrology》2022年第4期367-372,共6页亚洲男性学杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.81801518 and No.82071646).

摘　　要：The present study aimed to evaluate the clinical outcomes of magnetic-activated cell sorting(MACS)in sperm preparation for male subjects with a sperm DNA fragmentation index(DFI)≥30%.A total of 86 patients who had undergone their first long-term long protocol were selected.The protocol involved in vitro fertilization(IVF)and intracytoplasmic sperm injection(ICSI)cycles,and the patients were divided into the MACS or control groups.The MACS group included sperm samples analyzed with MACS that were combined with density gradient centrifugation(DGC)and the swim-up(SU)technique(n=39),and the control group included sperm samples prepared using standard techniques(DGC and SU;n=41).No differences were noted with regard to basic clinical characteristics,number of oocytes retrieved,normal fertilization rate,cleavage rate,or transplantable embryo rate between the two groups in IVF/ICSI.In addition,the clinical pregnancy and implantation rates of the first embryo transfer cycles indicated no significant differences between the two groups.However,there was a tendency to improve the live birth rate(LBR)of the first embryo transfer cycle(63.2%vs 53.9%)and the cumulative LBR(79.5%vs 70.7%)in the MACS group compared with the control group.Moreover,the number of transferred embryos(mean±standard deviation[s.d.]:1.7±0.7 vs 2.3±1.6)and the transfer number of each retrieved cycle(mean±s.d.:1.2±0.5 vs 1.6±0.8)were significantly lower in the MACS group than those in the control group.Thus,the selection of nonapoptotic spermatozoa by MACS for higher sperm DFI could improve assisted reproductive clinical outcomes.

关 键 词：cumulative live birth rate fertility rate intracytoplasmic sperm injection sperm DNA fragmentation index sperm DNA integrity 

分 类 号：R697[医药卫生—泌尿科学]