Metabolic enzyme gene polymorphisms predict the effects of antioxidant treatment on idiopathic male infertility  

作　　者：Hong-Yan Zhang Yi Mu Pan Chen Dong-Dong Liu Ke-Hang Chen Qi Yu Jun He Fa Sun Jun-Ping Xing Kai-Fa Tang 

机构地区：[1]Department of Respiratory Medicine,The Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China [2]Department of Urology,The Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China [3]Department of Urology,The Sixth's Hospital of Guiyang of Guizhou Province,Guiyang 550005,China [4]Department of Urology,People's Hospital of Guizhou Province,Guiyang 550002,China [5]Department of Urology,The First Affiliated Hospital of Medical College of Xi'an Jiaotong University,Xi'an 710061,China [6]Institute of Medical Science of Guizhou Medical University,Guiyang 550004,China

出　　处：《Asian Journal of Andrology》2022年第4期430-435,共6页亚洲男性学杂志（英文版）

基　　金：supported by the National Natural Science Fund of China(No.81660263 and No.81300541);the China Postdo.ctoral Science Foundation(No.2015M582760XB);the Doctoral Fund of Affiliated Hospital of Guiyang Medical College(No.C-2012-6).

摘　　要：To explore the relationship between genetic polymorphisms of metabolic enzymes such as CYP1A1,CYP2D6,GSTM1,GSTT1,and GSTP1 and idiopathic male infertility.By observing the efficacy of antioxidants in the treatment of idiopathic male infertility,the effect of metabolic enzyme gene polymorphisms on antioxidant therapy in patients with idiopathic male infertility was prospectively studied.This case-control study included 310 men with idiopathic infertility and 170 healthy controls.The cytochrome P4501A1(CYP1A1),cytochrome P4502D6(CYP2D6),glutathione S-transferase M1(GSTM1),glutathione S-transferase T1(GSTT1),and glutathione S-transferase P1(GSTP1)genotypes in peripheral blood samples were analyzed by polymerase chain reaction(PCR)and PCR-restriction fragment length polymorphism(PCR-RFLP).The idiopathic male infertility group was treated with vitamin C,vitamin E,and coenzyme Q10 for 3 months and followed up for 6 months.GSTM1(-),GSTT1(-),and GSTM1/T1(-/-)in the idiopathic male infertility groups were more common than those in the control group.The sperm concentration,motility,viability,mitochondrial membrane potential(MMP),and seminal plasma total antioxidant capacity(T-AOC)level in patients with GSTM1(-),GSTT1(-),and GSTM1/T1(-/-)were lower than those in wild-type carriers,and the sperm DNA fragmentation index(DFI),8-hydroxy-2'-deoxyguanosine(8-OH-dG),and malondialdehyde(MDA)and nitric oxide(NO)levels were higher.Therefore,oxidative damage may play an important role in the occurrence and development of idiopathic male infertility,but antioxidant therapy is not effective in male infertility patients with GSTM1 and GSTT1 gene deletions.

关 键 词：GENE INFERTILITY metabolic enzyme oxidative stress POLYMORPHISMS 

分 类 号：R697[医药卫生—泌尿科学]