黑龙江某地区3个规模化牛场BVDV qRT-PCR和MAP的ELISA检测报告  

ELISA Detection of BVDV qRT-PCR and MAP in Three Large-scale Cattle Farms in Heilongjiang Province

作　　者：金鑫 赵志博 黄宝银 朱战波[1] 刘宇[1] JIN Xin ZHAO Zhi-bo;HUANG Bao-yin;ZHU Zhan-bo;LIU Yu(College ofAnimal Science and Velerinary Medicine,HeiLongJiang BYiAgriculural Universily,Daqing,163319,China;Heilongiang Academy ofAgricultural Sciences Animal Husbandry and Velerinary Branch)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]黑龙江省农业科学院畜牧兽医分院,黑龙江齐齐哈尔161000

出　　处：《当代畜牧》2022年第5期23-25,共3页Contemporary Animal Husbandry

基　　金：NCP型BVDV急性感染中BTLA调控CD8+TLs活化、增殖及杀伤功能的机制研究,编号32072896。

摘　　要：为了对黑龙江某地区3个规模化牛场进行牛病毒性腹泻病毒和副结核分枝杆菌抗体的检测,某规模化牛场送检了900份牛耳组织与血清样本。首先,对来自A、B、C3个牛场的900份牛耳组织样品进行BVDV的qRT-PCR的检测。然后,用ELISA试剂盒检测副结核分枝杆菌抗体。结果表明,所有样品的BVDV检测阳性率为0。值得注意的是,2019年该地区3个牛场BVDV抗原检测平均阳性率为0.31%。经过1年的检疫,3个牛场均实现了对牛病毒性腹泻/黏膜病的净化。此外,牛副结核分枝杆菌抗体总体阳性率为3.33%,其中A场为2.86%、B场为2.48%、C场为4.36%。结果表明,在黑龙江某些地区仍存在牛副结核分枝杆菌感染的情况。In order to delect the antibodies of bovine viral diarhea virus and Mycobacterium paratuberculosis in three large-scale cattlefarms ina certain area of Heilongjiang Province,900 samples of bovine ear lissues and serum were sent to a large-scale catle farm forinspection.Firstly,900 bovine ear lissue samples from three cattle farmsA,Band C were delected by qRT-PCR of BVDV.Then,theantibody of Mycobacterium paratuberculosis was delecled by ELISA kit.The results showed that the positive rale of BVDV detection inall samples was O.ltis worth noting that the average positive rate of BVDV antigen detection in three cattle farms in this area was 0.31%in 2019.After a year of quarantine and purification,all three cattle farms achieved the purification of bovine viral dianhea/mucosal dis-ease.In addition the overall positive rale of antibody againsl mycobacterium paratuberculosis in cattle was 3.33%,of which 2.86%infield A,2.48%in field Band4.36%in field C.The results showed that Mycobacterium paratuberculosis infection still existed in someareas of Heilongjiang Province.

关 键 词：牛病毒性腹泻病毒 牛副结核分枝杆菌 QRT-PCR ELISA 检疫净化 

分 类 号：S854.43[农业科学—临床兽医学] S858.235.1[农业科学—兽医学] S858.235.3[农业科学—畜牧兽医]