4株丁酸梭菌的抗逆特性体外评估  

作　　者：胡乃志 焦国宝 马壮壮 马文锋[1] 廖家辉 王占彬[1] 陈晓宇 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471023 [2]河南崤函生物科技有限公司,河南三门峡472400

出　　处：《当代畜牧》2022年第5期48-51,共4页Contemporary Animal Husbandry

基　　金：中原科技创业领军人才计划(214300510016);河南科技大学大学生研究训练计划(2021379);河南省产学研合作项目(2018CX1012)。

摘　　要：笔者通过模拟饲料制粒过程及家禽胃肠道环境,比较4株丁酸梭菌抗逆性能及生长能力的差异。选择4株丁酸梭菌(编号CB1~CB4)分别与基础饲粮混合制成试验日粮,试验日粮经模拟制粒温度.人工胃液、人工胰液、人工胆盐的耐受性以及丁酸产量等方面进行比较研究。结果显示,4株丁酸梭菌在模拟制粒温度下处理2.5 min,存活率均在30%以上,其中菌株CB1耐热性最佳,存活率为61.55%;耐人工胃液试验中,pH=2.0处理48 min后,菌株CB2的耐受性最好、CB1次之,存活率分别为74.07%和70.37%;耐消化道试验中,菌株CB1对人工胰液的耐受性最好,存活率达92.75%,在胆盐浓度为0.3g/L的环境中,菌株CB4对胆盐的耐受性最好,存活率达到58.82%。综上所述,将4株丁酸梭菌进行比较,菌株CB1的性能更优。本试验结果为4株丁酸梭菌的生产性能评价提供了参考依据。(objective)In this experiment,the stress resistance of 4 strains of Clostridium butyicum were compared by simulating the feed plleing process and the gatroinesinal enironment of poultry.(Methods)Four strains of Clostridium butyricum(no.CB1 to CB4)were mixed with the basal diet to prepare experimental diets,then the experimental diets were compared in terms of simulated granula-tion temperature,arificial gastric juice,arifcial pancreatic juice,arificial bile salt tolerance and bulyric acid yield.(Resuls)The re-sults showed that the survival rales of the four strains of Clostridium butyricum treated at simulated granulation temperature for 2.5 min were more than 30%,of which strain CB1 had the best heat resistance and the survival rate was 61.55%;in the artificial gastric juice re-sistance test,after 48 min treatment with pH 2.0,the tolerance of strain CB2 was the best,followed by CB1,and the survival rales were 74.07%and 70.37%respectively;in the digestive tract tolerance test,strain CB1 had the best tolerance to artificial pancreatic juice,with a survival rate of 92.75%;in the bile salt concentration of 0.3 g/L,strain CB4 had the best tolerance to bile salt,with a survival rale of 58.82%.(Conclusion)In conclusion,compared with the four strains of Clostridium bulyricum,the performance of strain CB1 is bel-ter.The resuls of this test provide a reference basis for the ev aluation of four Clostridium butyricum strains.

关 键 词：丁酸梭菌 抗逆性 生长曲线 产酸力. 

分 类 号：S816.79[农业科学—饲料科学]