16S rRNA基因序列用于临床非典型细菌的补充鉴定  

Application of 16S rRNA gene sequence in the classification and identification of clinical atypical bacteria

作　　者：白志鹏[1] 曹美娜 张娜[3] 陈开廷 马静 刘玉婷 马婧 高金亮[2] BAI Zhi-peng;CAO Mei-na;ZHANG Na(Department of Medical Equipment,Ordos Central Hospital,Ordos 017000,China)

机构地区：[1]鄂尔多斯市中心医院器械科,内蒙古自治区鄂尔多斯市017000 [2]鄂尔多斯市中心医院分子医学实验室,内蒙古自治区鄂尔多斯市017000 [3]鄂尔多斯市中心医院检验科,内蒙古自治区鄂尔多斯市017000

出　　处：《中国实验诊断学》2022年第6期914-917,共4页Chinese Journal of Laboratory Diagnosis

基　　金：鄂尔多斯市产业创新创业人才团队项目(鄂人才办字[2016]11号)。

摘　　要：目的利用16SrRNA基因序列分析方法鉴定临床非典型细菌,为临床细菌分类鉴定新方法提供补充。方法提取细菌的DNA,自行设计引物并利用PCR技术扩增细菌16SrRNA基因片段,并对扩增片段进行序列测定,利用Blast等在线软件进行序列同源性分析。结果在32株临床非典型细菌中,3株细菌可以鉴定到“属”,29株细菌可以鉴定到“种”,其中2株可以鉴定到“亚种”。结论16SrRNA基因序列分析法是有效的病原细菌分类鉴定的补充。Objective To identify the clinical atypical bacteria by 16SrRNA gene sequence analysis for classification and diagnosis of bacteria rapidly and accurately.Methods The bacteria DNA was extracetd,followed by PCR amplication of 16SrRNA genes using self-designed primers,and the PCR products were sequenced.Blast and other databases were then used for sequence homology analysis.Results Among 32atypical bacteria strains,3could be specified to genus,29to species,and 2of the 29species could be further classified to subspecies level.Conclusion 16SrRNA gene sequence analysis is an effective complement to the classification and identification of pathogenic bacteria.

关 键 词：16S rRNA基因序列分析法 细菌分类 诊断 

分 类 号：R372[医药卫生—病原生物学]