菌剂混施对紫花苜蓿生长和土壤性质的影响  

Effects of mixed microbial agents on alfalfa growth and soil properties

作　　者：黄丽华 孔涛 张开 狄军贞[2] 王翼翔 张加良 HUANG Li-hua;KONG Tao;ZHANG Kai;DI Jun-zhen;WANG Yi-xiang;ZHANG Jia-liang(College of Environmental Science and Engi-neering,Liaoning Technical University,Fuxin 123000,Liaoning,China;School of Civil Engineering,Liaoning Technical University,Fuxin 123000,Liaoning,China)

机构地区：[1]辽宁工程技术大学环境科学与工程学院,辽宁阜新123000 [2]辽宁工程技术大学土木工程学院,辽宁阜新123000

出　　处：《生态学杂志》2022年第8期1580-1587,共8页Chinese Journal of Ecology

基　　金：辽宁工程技术大学学科创新团队资助项目(LNTU20TD-21);国家自然科学基金面上项目(41672247);辽宁省“兴辽英才”青年拔尖人才计划支持项目(XLYC1807159)资助。

摘　　要：为了明确哈茨木霉菌(TH)、胶质芽孢杆菌(BM)与摩西球囊霉菌(GM)在土壤改良中的协同作用,设置单施处理、双菌混施处理、三菌混施处理及对照共8个处理,进行盆栽试验,测定紫花苜蓿生长指标、土壤养分含量和土壤微生物指标。结果表明:各处理均能显著提高紫花苜蓿的生长指标,其中以TH+BM双菌混施为效果最优,生物量比对照显著提高93.8%。在土壤养分和微生物方面,单施处理中BM对土壤综合改良效果最佳,能够显著提高土壤速效磷、碱解氮、速效钾含量和微生物生物量氮,比对照显著提高58.1%、8.9%、13.1%和77.6%;双菌混施处理中,TH+BM双菌混施对土壤综合改良效果最佳,对速效磷、碱解氮、速效钾含量和微生物生物量碳、氮具有显著的协同作用,分别比对照提高93.1%、26.7%、23.1%、78.7%、143.3%;TH+BM+GM三菌混施对土壤基质的改良效果介于3种双菌混施处理之间。综合来说,双菌混施处理对土壤综合改良的效果好于单施和三菌混施,TH+BM双菌混施对土壤综合改良效果最佳,可以作为土壤质量提升的一种良好的改良技术。To clarify the synergistic effects of Trichoderma harzianum(TH),Bacillus mucilaginosus(BM),and Glomus mosseae(GM) on soil quality improvement,three single application treatments,three two-bacterium mixed application treatments,one three-bacterium mixed application treatment and control treatment were set up.The growth index of alfalfa,soil nutrient content and soil microbial index were measured in a pot experiment.The results showed that the growth indices of alfalfa were significantly improved by single application and mixed application.The application of TH+BM had the strongest effect,with the biomass of alfalfa being significantly increased by 93.8% compared with that in the control.In terms of soil nutrients and microorganisms,among the single application treatments,BM treatment had the best comprehensive improvement effect on soil nutrients,increasing the contents of available P,available N,available K and microbial biomass nitrogen(MBN) by 58.1%,8.9%,13.1% and 77.6% respectively.Among the two-bacterium mixed application treatments,TH+BM had the strongest effect on soil comprehensive improvement,and increased available P,available N,available K contents,microbial biomass C and MBN by 93.1%,26.7%,23.1%,78.7%,143.3% respectively.The effect of the three-bacterium mixed application on soil substrate improvement was within the range of the three two-bacterium mixed application treatments.In general,the effects of two-bacterium mixed application on soil comprehensive improvement were better than that of single application and three-bacterium mixed application.The TH+BM treatment had the best effect,which could be used for soil quality improvement.

关 键 词：哈茨木霉菌 胶质芽孢杆菌 摩西球囊霉菌 菌剂混施 

分 类 号：S541.9[农业科学—作物学]