杭州市家蝇高效氯氰菊酯抗性品系的建立  

Establishment of beta-cypermethrin-resistant strains of Musca domestica in Hangzhou

作　　者：韦凌娅[1] 孔庆鑫[1] 曹阳 王慧敏 沈林海[1] 王英红[1] WEI Ling-ya;KONG Qing-xin;CAO Yang;WANG Hui-min;SHEN Lin-hai;WANG Ying-hong(Department of Disinfection Surveillance and Vector Control,Hangzhou Center for Disease Control and Prevention,Hangzhou,Zhejiang 310021,China)

机构地区：[1]杭州市疾病预防控制中心消毒监测与病媒生物防治所,浙江杭州310021

出　　处：《中国媒介生物学及控制杂志》2022年第4期458-461,共4页Chinese Journal of Vector Biology and Control

基　　金：浙江省卫生健康科技计划(2018KY637);杭州市医学重点学科(消毒与媒介生物控制学)项目。

摘　　要：目的对杭州市家蝇野外种群进行高效氯氰菊酯高抗稳定品系的筛选与纯化。方法采集杭州市不同方位的家蝇,用高效氯氰菊酯直接喷雾法筛选家蝇成蝇,选育5代后进行维持饲养2年。采用微量点滴法测定家蝇F2代对溴氰菊酯、高效氯氰菊酯、敌敌畏、三氯杀虫酯、残杀威的抗药性;测定家蝇F7代对溴氰菊酯、高效氯氰菊酯的抗药性;测定家蝇维持饲养1年与2年时对溴氰菊酯、高效氯氰菊酯的抗药性。敏感品系抗药性测试数据由浙江省疾病预防控制中心传染病预防控制所病媒生物防制科提供。结果杭州市家蝇野外种群F2代(雌蝇)对5种常用杀虫剂均已产生抗性,残杀威的LD_(50)>400.0000μg/只,抗性倍数>1345.00,抗性水平最高,对其他杀虫剂的抗性倍数在6.79~77.33,其中对溴氰菊酯的抗性为77.33倍(LD_(50)=0.0696μg/只),对高效氯氰菊酯抗性为55.58倍(LD_(50)=0.2001μg/只)。进一步用高效氯氰菊酯连续5代筛选后,F7代与F2代相比,对溴氰菊酯和高效氯氰菊酯的抗性分别升高29.89%和51.27%。经过2年正常饲养,杭州市家蝇高效氯氰菊酯抗性品系抗药性表型较F7代有一定下降,但与敏感品系相比,对溴氰菊酯和高效氯氰菊酯的抗性分别为70.22和56.25倍,仍处于高抗水平。整个筛选过程中残杀威始终LD_(50)>400.0000μg/只。结论经过高效氯氰菊酯连续5代的筛选和2年的维持饲养,获得较为稳定的杭州市家蝇高效氯氰菊酯抗性品系,为进一步研究家蝇的抗药性机制奠定了基础。Objective To screen and purify stable strains highly resistant to beta-cypermethrin using the field samples of Musca domestica in Hangzhou,China.Methods Houseflies were collected from different locations in Hangzhou,and adult houseflies were screened by direct spray with beta-cypermethrin.After screening for 5 generations,the houseflies were maintained for two years.Resistance was determined using topical application method for F2 against deltamethrin,beta-cypermethrin,dichlorvos,triclosan,and propoxur,for F7 against deltamethrin and beta-cypermethrin,and for houseflies maintained for 1 and 2 years against deltamethrin and beta-cypermethrin.The resistance data of sensitive strains were provided by the department of infectious diseases,Zhejiang provincial center for disease control and prevention.Results The F2 generation of M.domestica in Hangzhou developed resistance to the five commonly used insecticides.The LD_(50)of propoxur was>400.0000μg/♀,with the highest resistance and R/S>1345.00.Resistance to other insecticide showed R/S between 6.79 and 77.33.The R/Sfor deltamethrin was 77.33(LD_(50)=0.0696μg/♀)and the R/S for beta-cypermethrin was 55.58(LD_(50)=0.2001μg/♀).After being screened with beta-cypermethrin for 5 consecutive generations,compared with F2,F7 showed 29.89% and 51.27% increases in resistance to deltamethrin and betacypermethrin,respectively.After 2 years of normal rearing,the beta-cypermethrin-resistant strain showed decreased resistance as compared with the F7 generation;however,compared with sensitive strains,the R/S values for deltamethrin and beta-cypermethrin were 70.22 and 56.25,respectively,and the resistance remained at high levels.The LD_(50)for propoxur remained above 400.0000μg/♀.Conclusion After being screened with beta-cypermethrin for 5 generations and maintaining for two years,relatively stable beta-cypermethrin-resistant strains of M.domestica in Hangzhou were obtained,which lays a foundation for further research on the resistance mechanism of M.domestica.

关 键 词：家蝇 抗药性 杀虫剂 

分 类 号：R384.2[医药卫生—医学寄生虫学] Q965.9[医药卫生—基础医学]